AUTHOR=Ingegnere Tiziano , Mariotti Francesca Romana , Pelosi Andrea , Quintarelli Concetta , De Angelis Biagio , Tumino Nicola , Besi Francesca , Cantoni Claudia , Locatelli Franco , Vacca Paola , Moretta Lorenzo 

TITLE=Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

JOURNAL=Frontiers in Immunology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2019.00957

DOI=10.3389/fimmu.2019.00957

ISSN=1664-3224

ABSTRACT=<p>CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.</p>