AUTHOR=Mendy Joseph , Jarju Sheikh , Heslop Rhiannon , Bojang Adama L. , Kampmann Beate , Sutherland Jayne S. 

TITLE=Changes in Mycobacterium tuberculosis-Specific Immunity With Influenza co-infection at Time of TB Diagnosis

JOURNAL=Frontiers in Immunology

VOLUME=9

YEAR=2019

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.03093

DOI=10.3389/fimmu.2018.03093

ISSN=1664-3224

ABSTRACT=<p><bold>Background:</bold> Prior Influenza A viral (IAV) infection has been shown to increase susceptibility to tuberculosis (TB) and TB has also been shown to be a primary cause of death during pandemics, including the Spanish Influenza outbreak of 1918–1919. The majority of data has been obtained from mouse models, thus the aim of this study was to determine the impact of Flu co-infection on host immunity and disease severity in TB patients at diagnosis.</p><p><bold>Methods:</bold> Sputum from 282 patients with active TB were analyzed for presence of FluA/FluB RNA at presentation using multiplex PCR. Sputum RNA was also analyzed for <italic>Mycobacterium tuberculosis</italic> (Mtb) load using <sup>16</sup>S RNA amplification. Supernatants from digested sputum and Mtb antigen-stimulated whole blood were analyzed using multiplex cytokine arrays and PBMC were analyzed for cytokine production from CD4+ T, CD8+ T and Mucosal Associated Invariant T cells (MAITs).</p><p><bold>Results:</bold> 12 (4.3%) of TB patients were found to have FluA or FluB viral RNA present in their sputum at the time of TB diagnosis. The TB/Flu co-infected patients had a significantly higher bacterial load compared to those with TB mono-infection (<italic>p</italic> = 0.0026). They had lower levels of IL17A in <italic>ex vivo</italic> sputum (<italic>p</italic> = 0.0275) and higher MCP-1 (CCL2) levels in the blood following PPD stimulation (<italic>p</italic> = 0.0267). TB/Flu co-infected subjects had significantly higher IFN-γ+IL-17+CD4+ and IFN-γ+IL-17-CD8+ cells compared to TB mono-infected subjects.</p><p><bold>Conclusions:</bold> These data show that Flu co-infection at time of TB diagnosis is associated with a higher bacterial load and differential cellular and soluble profiles. These findings show for the first time the impact of TB/Flu co-infection in a human cohort and support the potential benefit of Flu vaccination in TB-endemic settings.</p>