AUTHOR=De Zuani Marco , Paolicelli Giuseppe , Zelante Teresa , Renga Giorgia , Romani Luigina , Arzese Alessandra , Pucillo Carlo E. M. , Frossi Barbara 

TITLE=Mast Cells Respond to Candida albicans Infections and Modulate Macrophages Phagocytosis of the Fungus

JOURNAL=Frontiers in Immunology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.02829

DOI=10.3389/fimmu.2018.02829

ISSN=1664-3224

ABSTRACT=<p>Mast cells (MCs) are long-lived immune cells widely distributed at mucosal surfaces and are among the first immune cell type that can get in contact with the external environment. This study aims to unravel the mechanisms of reciprocal influence between mucosal MCs and <italic>Candida albicans</italic> as commensal/opportunistic pathogen species in humans. Stimulation of bone marrow-derived mast cells (BMMCs) with live forms of <italic>C. albicans</italic> induced the release of TNF-α, IL-6, IL-13, and IL-4. Quite interestingly, BMMCs were able to engulf <italic>C. albicans</italic> hyphae, rearranging their α-tubulin cytoskeleton and accumulating LAMP1<sup>+</sup> vesicles at the phagocytic synapse with the fungus. <italic>Candida</italic>-infected MCs increased macrophage crawling ability and promoted their chemotaxis against the infection. On the other side, resting MCs inhibited macrophage phagocytosis of <italic>C. albicans</italic> in a contact-dependent manner. Taken together, these results indicate that MCs play a key role in the maintenance of the equilibrium between the host and the commensal fungus <italic>C. albicans</italic>, limiting pathological fungal growth and modulating the response of resident macrophages during infections.</p>