AUTHOR=Zhou Xiu-Man , Li Wan-Qiong , Wu Ya-Hong , Han Lu , Cao Xin-Guang , Yang Xuan-Ming , Wang Hong-Fei , Zhao Wen-Shan , Zhai Wen-Jie , Qi Yuan-Ming , Gao Yan-Feng TITLE=Intrinsic Expression of Immune Checkpoint Molecule TIGIT Could Help Tumor Growth in vivo by Suppressing the Function of NK and CD8+ T Cells JOURNAL=Frontiers in Immunology VOLUME=9 YEAR=2018 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.02821 DOI=10.3389/fimmu.2018.02821 ISSN=1664-3224 ABSTRACT=
TIGIT, an immune checkpoint molecule widely expressed on NK cells, activated T cells and Tregs, has been involved in delivering inhibitory signals through the interaction with PVR. The blockade of TIGIT/PVR interaction is a promising approach in cancer immunotherapy. Here, we unexpectedly discovered the expression of TIGIT in murine tumor cells. To elucidate the mechanism of such intrinsic expression, TIGIT knockout murine colorectal CT26 and MC38 cell lines were generated by using CRISPR/Cas9 system. Although TIGIT knockout showed no effects on proliferation and colony formation of tumor cells