AUTHOR=Shen Juan , Luo Xi , Wu Qiongli , Huang Jun , Xiao Guanying , Wang Liantang , Yang Binyan , Li Huabin , Wu Changyou TITLE=A Subset of CXCR5+CD8+ T Cells in the Germinal Centers From Human Tonsils and Lymph Nodes Help B Cells Produce Immunoglobulins JOURNAL=Frontiers in Immunology VOLUME=9 YEAR=2018 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.02287 DOI=10.3389/fimmu.2018.02287 ISSN=1664-3224 ABSTRACT=

Recent studies indicated that CXCR5⁺CD8⁺ T cells in lymph nodes could eradicate virus-infected target cells. However, in the current study we found that a subset of CXCR5⁺CD8⁺ T cells in the germinal centers from human tonsils or lymph nodes are predominately memory cells that express CD45RO and CD27. The involvement of CXCR5⁺CD8⁺ T cells in humoral immune responses is suggested by their localization in B cell follicles and by the concomitant expression of costimulatory molecules, including CD40L and ICOS after activation. In addition, CXCR5⁺CD8⁺ memory T cells produced significantly higher levels of IL-21, IFN-γ, and IL-4 at mRNA and protein levels compared to CXCR5⁻CD8⁺ memory T cells, but IL-21-expressing CXCR5⁺CD8⁺ T cells did not express Granzyme B and perforin. When cocultured with sorted B cells, sorted CXCR5⁺CD8⁺ T cells promoted the production of antibodies compared to sorted CXCR5⁻CD8⁺ T cells. However, fixed CD8⁺ T cells failed to help B cells and the neutralyzing antibodies against IL-21 or CD40L inhibited the promoting effects of sorted CXCR5⁺CD8⁺ T cells on B cells for the production of antibodies. Finally, we found that in the germinal centers of lymph nodes from HIV-infected patients contained more CXCR5⁺CD8⁺ T cells compared to normal lymph nodes. Due to their versatile functional capacities, CXCR5⁺CD8⁺ T cells are promising candidate cells for immune therapies, particularly when CD4⁺ T cell help are limited.