AUTHOR=Lee Sung Hwan , Park Sang A , Zou Yunyun , Seo Sang-Uk , Jun Chang-Duk , Lee Woo Jung , Hyun Young-Min , Cho Nam Hoon 

TITLE=Real-Time Monitoring of Cancer Cells in Live Mouse Bone Marrow

JOURNAL=Frontiers in Immunology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.01681

DOI=10.3389/fimmu.2018.01681

ISSN=1664-3224

ABSTRACT=<p>Disseminated tumor cells in the bone marrow environment are the main cause of systemic metastasis after curative treatment for major solid tumors. However, the detailed biological processes of tumor biology in bone marrow have not been well defined in a real-time manner, because of a lack of a proper <italic>in vivo</italic> experimental model thereof. In this study, we established intravital imaging models of the bone marrow environment to enable real-time observation of cancer cells in the bone marrow. Using these novel imaging models of intact bone marrow and transplanted bone marrow of mice, respectively, <italic>via</italic> two-photon microscopy, we could first successfully track and analyze both the distribution and the phenotype of cancer cells in bone marrow of live mouse. Therefore, these novel <italic>in vivo</italic> imaging models for the bone marrow would provide a valuable tool to identify the biologic processes of cancer cells in a real-time manner in a live animal model.</p>