AUTHOR=Caldirola María Soledad , Rodríguez Broggi María Guadalupe , Gaillard María Isabel , Bezrodnik Liliana , Zwirner Norberto Walter TITLE=Primary Immunodeficiencies Unravel the Role of IL-2/CD25/STAT5b in Human Natural Killer Cell Maturation JOURNAL=Frontiers in Immunology VOLUME=9 YEAR=2018 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.01429 DOI=10.3389/fimmu.2018.01429 ISSN=1664-3224 ABSTRACT=

Natural killer (NK) cells play a pivotal role during immunity against viruses and circumstantial evidence also indicates that they can protect the host against developing tumors. Peripheral blood NK cells comprise CD56^brightCD16^lo/− cells that constitutively express CD25 (IL-2Rα) and CD56^dimCD16^hi cells that express CD25 upon activation. Using NK cells from two patients, one with a primary immunodeficiency characterized by a homozygous mutation in CD25 (born in year 2007 and studied since she was 3 years old) and one with a homozygous mutation in STAT5b (born in year 1992 and studied since she was 10 years old), we observed that the absence of IL-2 signaling through CD25 promotes the accumulation of CD56^brightCD16^high NK cells, and that CD56^brightCD16^lo, CD56^brightCD16^high, and CD56^dimCD16^high NK cells of this patient exhibited higher content of perforin and granzyme B, and proliferation capacity, compared to healthy donors. Also, CD56^bright and CD56^dim NK cells of this patient exhibited a reduced IFN-γ production in response to cytokine stimulation and increased degranulation against K562 cells. Also, the CD25-deficient patient presented a lower frequency of terminally differentiated NK cells in the CD56^dimCD16^hi NK subpopulation compared to the HD (assessed by CD57 and CD94 expression). Remarkably, CD56^dimCD16^high NK cells from both patients exhibited notoriously higher expression of CD62L compared to HD, suggesting that in the absence of IL-2 signaling through CD25 and STAT5b, NK cells fail to properly downregulate CD62L during their transition from CD56^brightCD16^lo/− to CD56^dimCD16^hi cells. Thus, we provide the first demonstration about the in vivo requirement of the integrity of the IL-2/CD25/STAT5b axis for proper human NK cell maturation.