AUTHOR=Rouet Romain , Jackson Katherine J. L. , Langley David B. , Christ Daniel 

TITLE=Next-Generation Sequencing of Antibody Display Repertoires

JOURNAL=Frontiers in Immunology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.00118

DOI=10.3389/fimmu.2018.00118

ISSN=1664-3224

ABSTRACT=<p><italic>In vitro</italic> selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we discuss recent examples relating to the use of NGS to assess library diversity, clonal enrichment, and affinity maturation.</p>