AUTHOR=Tay Neil Q. , Lee Debbie C. P. , Chua Yen Leong , Prabhu Nayana , Gascoigne Nicholas R. J. , Kemeny David M. TITLE=CD40L Expression Allows CD8+ T Cells to Promote Their Own Expansion and Differentiation through Dendritic Cells JOURNAL=Frontiers in Immunology VOLUME=8 YEAR=2017 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2017.01484 DOI=10.3389/fimmu.2017.01484 ISSN=1664-3224 ABSTRACT=

CD8⁺ T cells play an important role in providing protective immunity against a wide range of pathogens, and a number of different factors control their activation. Although CD40L-mediated CD40 licensing of dendritic cells (DCs) by CD4⁺ T cells is known to be necessary for the generation of a robust CD8⁺ T cell response, the contribution of CD8⁺ T cell-expressed CD40L on DC licensing is less clear. We have previously shown that CD8⁺ T cells are able to induce the production of IL-12 p70 by DCs in a CD40L-dependent manner, providing some evidence that CD8⁺ T cell-mediated activation of DCs is possible. To better understand the role of CD40L on CD8⁺ T cell responses, we generated and characterized CD40L-expressing CD8⁺ T cells both in vitro and in vivo. We found that CD40L was expressed on 30–50% of effector CD8⁺ T cells when stimulated and that this expression was transient. The expression of CD40L on CD8⁺ T cells promoted the proliferation and differentiation of both the CD40L-expressing CD8⁺ T cells and the bystander effector CD8⁺ T cells. This process occurred via a cell-extrinsic manner and was mediated by DCs. These data demonstrate the existence of a mechanism where CD8⁺ T cells and DCs cooperate to maximize CD8⁺ T cell responses.