AUTHOR=Solf Benjamin , Schramm Stefan , Blum Maren-Christina , Klee Sascha 

TITLE=The Influence of the Stimulus Design on the Harmonic Components of the Steady-State Visual Evoked Potential

JOURNAL=Frontiers in Human Neuroscience

VOLUME=14

YEAR=2020

URL=https://www.frontiersin.org/journals/human-neuroscience/articles/10.3389/fnhum.2020.00343

DOI=10.3389/fnhum.2020.00343

ISSN=1662-5161

ABSTRACT=<p>Steady-state visual evoked potentials (ssVEPs) are commonly used for functional objective diagnostics. In general, the main response at the stimulation frequency is used. However, some studies reported the main response at the second harmonic of the stimulation frequency. The aim of our study was to analyze the influence of the stimulus design on the harmonic components of ssVEPs. We studied 22 subjects (8 males, mean age ± SD = 27 ± 4.8 years) using a circular layout (<italic>r</italic><sub>1</sub> = 0–1.6°, <italic>r</italic><sub>2</sub> = 1.6–3.5°, <italic>r</italic><sub>3</sub> = 3.5–6.4°, <italic>r</italic><sub>4</sub> = 6.4–10.9°, and <italic>r</italic><sub>5</sub> = 10.9–18°). At a given eccentricity, the stimulus was presented according to a 7.5 Hz square wave with 50% duty cycle. To analyze the influence of the stimulus eccentricity, a background luminance of 30 cd/m<sup>2</sup> was added to suppress foveal stray light effects; to analyze the influence of simultaneous foveal and peripheral stimulations, stimulations are performed without stray light suppression. For statistical analysis, medians <bold>M</bold> of the amplitude ratios for amplitudes at the second harmonic to the first harmonic and the probability of the occurrence of the main response at the second harmonic <bold>P(MCSH)</bold> are calculated. For stimulations with foveal stray light suppression, the medians were <bold>M</bold><sub><bold>0</bold>–<bold>1.6</bold>°</sub> = 0.45, <bold>M</bold><sub><bold>1.6</bold>–<bold>3.5</bold>°</sub> = 0.45, <bold>M</bold><sub><bold>3.5</bold>–<bold>6.4</bold>°</sub> = 0.76, <bold>M</bold><sub><bold>6.4</bold>–<bold>10.9</bold>°</sub> = 0.72, and <bold>M</bold><sub><bold>10.9</bold>–<bold>18</bold>°</sub> = 0.48, and the probabilities were <bold>P</bold><sub><bold>0–1.6</bold>°</sub><bold>(MCSH)</bold> = 0.05, <bold>P</bold><sub><bold>1.6</bold>–<bold>3.5</bold>°</sub><bold>(MCSH)</bold> = 0.05, <bold>P</bold><sub><bold>3.5</bold>–<bold>6.4</bold>°</sub><bold>(MCSH)</bold> = 0.32, <bold>P</bold><sub><bold>6.4</bold>–<bold>10.9</bold>°</sub><bold>(MCSH)</bold> = 0.29, and <bold>P</bold><sub><bold>10.9</bold>–<bold>18</bold>°</sub><bold>(MCSH)</bold> = 0.30. For stimulations without foveal stray light suppression, the medians <bold>M</bold> were <bold>M</bold><sub><bold>0</bold>–<bold>1.6</bold>°</sub> = 0.29, <bold>M</bold><sub><bold>1.6</bold>–<bold>3.5</bold>°</sub> = 0.37, <bold>M</bold><sub><bold>3.5</bold>–<bold>6.4</bold>°</sub> = 0.98, <bold>M</bold><sub><bold>6.4</bold>–<bold>10.9</bold>°</sub> = 1.08, and <bold>M</bold><sub><bold>10.9</bold>–<bold>18</bold>°</sub> = 1.24, and the probabilities were <bold>P</bold><sub><bold>0–1.6</bold>°</sub><bold>(MCSH)</bold> = 0.09, <bold>P</bold><sub><bold>1.6</bold>–<bold>3.5</bold>°</sub><bold>(MCSH)</bold> = 0.05, <bold>P</bold><sub><bold>3.5</bold>–<bold>6.4</bold>°</sub><bold>(MCSH)</bold> = 0.50, <bold>P</bold><sub><bold>6.4</bold>–<bold>10.9</bold>°</sub><bold>(MCSH)</bold> = 0.55, and <bold>P</bold><sub><bold>10.9</bold>–<bold>18</bold>°</sub><bold>(MCSH)</bold> = 0.55. In conclusion, the stimulus design has an influence on the harmonic components of ssVEPs. An increase in stimulation eccentricity during extrafoveal stimulation leads to a transition of the main response to the second harmonic. The effect is enhanced by a simultaneous foveal stimulation.</p>