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REVIEW article

Front. Genome Ed.
Sec. Genome Editing in Plants
Volume 6 - 2024 | doi: 10.3389/fgeed.2024.1490295
This article is part of the Research Topic Genome Editing for Addressing the Challenges of Climate Change Adaptation in Agriculture Crops and Livestock View all articles

Crop genome editing through tissue-culture-independent transformation methods

Provisionally accepted
Alejandro Sebiani-Calvo Alejandro Sebiani-Calvo 1Alejandro Hernández-Soto Alejandro Hernández-Soto 2Goetz Hensel Goetz Hensel 3Andres Gatica-Arias Andres Gatica-Arias 1*
  • 1 School of Biology, Faculty of Sciences, University of Costa Rica, San Pedro, Costa Rica
  • 2 Instituto Tecnológico de Costa Rica (ITCR), Cártago, Cartago, Costa Rica
  • 3 Heinrich Heine University of Düsseldorf, Düsseldorf, North Rhine-Westphalia, Germany

The final, formatted version of the article will be published soon.

    Genome editing and plant transformation are crucial techniques in plant biotechnology, allowing for the precise modification of plant genomes to enhance agronomically essential traits. The advancement of CRISPR-based genome editing tools in plants is limited, among others, by developing novel in vitro tissue culture methodologies for efficient plant genetic transformation. In-planta methodologies offer a promising alternative to overcome tissue culture limitations and facilitate crops' genetic improvement. The inplanta transformation methods can be categorized under the definition of means of plant genetic transformation with no or minimal tissue culture steps meeting the conditions for minimal steps: short duration with a limited number of transfers, high technical simplicity, limited list of hormones, and that the regeneration does not undergo callus development. In this review, we analyzed over 250 articles. We identified studies that follow an in-planta transformation methodology for delivering CRISPR/Cas9 components focusing on crop plants, as model species have been previously reviewed in detail. This approach has been successfully applied for genome editing in crop plants: camelina, cotton, lemon, melon, orange, peanut, rice, soybean, and wheat. Overall, this study underscores the importance of in-planta methodologies in overcoming the limitations of tissue culture and advancing the field of plant genome editing. Keywords agriculture • CRISPR • crop improvement • in-planta transformation • genome editing 1.

    Keywords: Agriculture, CRISPR, crop improvement 2, In-planta transformation, Genome editing

    Received: 02 Sep 2024; Accepted: 22 Nov 2024.

    Copyright: © 2024 Sebiani-Calvo, Hernández-Soto, Hensel and Gatica-Arias. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Andres Gatica-Arias, School of Biology, Faculty of Sciences, University of Costa Rica, San Pedro, Costa Rica

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.