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REVIEW article

Front. Genome Ed.
Sec. Genome Editing in Human Health and Disease
Volume 6 - 2024 | doi: 10.3389/fgeed.2024.1467449
This article is part of the Research Topic In vivo Therapeutic Approaches Based on Liver-Targeted Genome Editing View all articles

The potential of HBV cure: an overview of CRISPR-mediated HBV gene disruption

Provisionally accepted
Zhi Q. Yao Zhi Q. Yao *Madison B. Schank Madison B. Schank Juan Zhao Juan Zhao Mohamed El Gazzar Mohamed El Gazzar Ling Wang Ling Wang Yi Zhang Yi Zhang Addison C. Hill Addison C. Hill Puja Banik Puja Banik Jaeden S. Pyburn Jaeden S. Pyburn Jonathan P. Moorman Jonathan P. Moorman
  • East Tennessee State University, Johnson City, United States

The final, formatted version of the article will be published soon.

    This article reviewed the CRISPR/Cas9 technology to disrupt and inactivate HBV genome.We summarized the tools employed in designing guide RNAs (gRNAs) targeting HBV genomes, the vehicles used for expressing and delivering CRISPR/Cas9 components, the models used for evaluating CRISPR-mediated HBV gene disruption, the methods used for assessing antiviral and off-target effects induced by CRISPR/Cas9, and the prospects of future directions and challenges in leveraging this HBV gene-editing approach to advance the HBV treatment toward a clinical cure.

    Keywords: HBV, cccDNA, CRISPR/Cas9, gene editing, gene therapy 7-AAD, 7-Aminoactinomycin D, AAV, adeno-associated virus, AuNPs, gold nanoparticles, Av, annexin V, BE, base-editing, BWA, Burrows-Wheeler Aligner, CRISPR, clustered regularly interspaced short palindromic repeats, Cas9, CRISPR-associated protein 9

    Received: 19 Jul 2024; Accepted: 24 Sep 2024.

    Copyright: © 2024 Yao, Schank, Zhao, El Gazzar, Wang, Zhang, Hill, Banik, Pyburn and Moorman. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Zhi Q. Yao, East Tennessee State University, Johnson City, United States

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.