AUTHOR=Nonaka Satoko , Ito Maki , Ezura Hiroshi TITLE=Targeted modification of CmACO1 by CRISPR/Cas9 extends the shelf-life of Cucumis melo var. reticulatus melon JOURNAL=Frontiers in Genome Editing VOLUME=5 YEAR=2023 URL=https://www.frontiersin.org/journals/genome-editing/articles/10.3389/fgeed.2023.1176125 DOI=10.3389/fgeed.2023.1176125 ISSN=2673-3439 ABSTRACT=
The gaseous plant hormone ethylene is a regulator of fruit shelf-life, one of the essential traits in fruits. Extending fruit shelf-life reduces food loss, thereby expected to contribute to food security. The enzyme 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) is the final step of the ethylene production pathway. Its suppression via antisense technology has been demonstrated to extend the shelf-life of melon, apple, and papaya. Genome editing technology is an innovative technique for plant breeding. Because the genome editing technology would not leave the exogenous genes in the final crop products, the crops via genome editing can be considered non-genetically modified yields; compared to conventional breeding, such as mutation breeding, the breeding term would be expected to be relatively short. These points include the advantage of this technique in utilization for commercial applications. We attempted to extend the shelf-life of the Japanese luxury melon (