This article is a correction to:
Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research)
Frida Meijer Carlsen1†
Ida Elisabeth Johansen1,2†
Zhang Yang3
Ying Liu1,4Ida Nøhr Westberg1
Nam Phuong Kieu5
Bodil Jørgensen1Marit Lenman5
Erik Andreasson5
Kåre Lehmann Nielsen6
Andreas Blennow1
Bent Larsen Petersen1*- 1Department of Plant and Environmental Sciences, Faculty of Science, The University of Copenhagen, Copenhagen, Denmark
- 2Kartoffel Mel Centralen Amba, Brande, Denmark
- 3Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
- 4Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden
- 5Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, Sweden
- 6Bioscience, Aalborg University, Aalborg, Denmark
A Corrigendum on
Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research)
by Carlsen FM, Johansen IE, Yang Z, Liu Y, Westberg IN, Kieu NP, Jørgensen B, Lenman M, Andreasson E, Nielsen KL, Blennow A and Petersen BL (2022). Front. Genome Ed. 3:795644. doi: 10.3389/fgeed.2021.795644
In the original article, there were various errors present throughout the main text. These errors have been corrected in the original article.
Additionally, Figure 1 and Table 1 have been updated. The updated figure and table are shown below. The Funding statement has also been updated and is shown below.
FIGURE 1
FIGURE 1. Glucan Water Dikinase (GWD) 1—structure and full allelic sequence of gRNA target regions. Overall gene structure with exons (boxes) and the area containing carbohydrate-binding module (CBM) depicted above. Left: the nucleotide sequence of exon 1 and introns. Right: exon 24 and 25 including introns. Exons are depicted in capital letters with the amino acid sequence indicated. Small nucleotide polymorphisms (SNPs) from cultivars included in the SPUD database are marked with red, and SNPs found in Saturna are underlined. Grey arrows designate gRNAs (gA, gB, gC, gD, gE, gI, gJ, gK, gL, and gM) with PAM sites marked in bold. Red arrows designate diagnostic IDAA PCR primers. The “CFATC” region, containing cysteine’s hypothesized to be involved in inter or intra-di-sulfide bond formation and thus in putative redox-state modulation of GWD activity is marked with bold. The active site histidine residue is also marked with bold.
TABLE 1
TABLE 1. gRNAs and diagnostic IDAA primers for each of the four target regions. Scores and first selection of gRNAs were obtained by feeding ca 1 kb regions to the in silico prediction servers CHOPCHOP (http://chopchop.cbu.uib.no/), CRISPRater (https://crispr.cos.uniheidelberg.de/) and SSC (http://crispr.dfci.harvard.edu/SSC/).
Funding
Kartoffelafgiftfonden (2021) “Samtidig introduktion af flere egenskaber i kartoffel vha. DNA-fri CRISPR”. Kartoffelafgiftfonden (2022)” Reduceret miljø og klima aftryk gennem præcis forædling af skimmel resistens i stivelses kartofler”. Novo Nordisk Foundation (2020) “Gene edited potatoes for reduced pesticide usage” (NNF19OC0057208). Grønt Udviklings-og Demonstrations program (GUDP) (2020) “KRISPS Kartofler med Resistens og Innovativ Stivelse som Platform for Synergi mellem grøn og økonomisk bæredygtighed” (34009-19-1531). DFF/Independent Research Fund Denmark (2022) “Green CRISPR production platform for new starch qualities” (1032-00399B).”
The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way. The original article has been updated.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Keywords: ribonucleoprotein/gRNA design, gene editing (CRISPR/Cas9), protoplast, gRNA efficiency, multiplexing, complex genome, potato
Citation: Carlsen FM, Johansen IE, Yang Z, Liu Y, Westberg IN, Kieu NP, Jørgensen B, Lenman M, Andreasson E, Nielsen KL, Blennow A and Petersen BL (2022) Corrigendum: Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research). Front. Genome Ed. 4:914100. doi: 10.3389/fgeed.2022.914100
Received: 06 April 2022; Accepted: 21 June 2022;
Published: 04 August 2022.
Edited and
Reviewed by: Leena Tripathi, International Institute of Tropical Agriculture (IITA), KenyaCopyright © 2022 Carlsen, Johansen, Yang, Liu, Westberg, Kieu, Jørgensen, Lenman, Andreasson, Nielsen, Blennow and Petersen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Bent Larsen Petersen, blp@plen.ku.dk
†These authors have contributed equally to this work