AUTHOR=Wolff Jonas Holst , Haldrup Jakob , Thomsen Emil Aagaard , Andersen Sofie , Mikkelsen Jacob Giehm 

TITLE=piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

JOURNAL=Frontiers in Genome Editing

VOLUME=3

YEAR=2021

URL=https://www.frontiersin.org/journals/genome-editing/articles/10.3389/fgeed.2021.786893

DOI=10.3389/fgeed.2021.786893

ISSN=2673-3439

ABSTRACT=<p>Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.</p>