AUTHOR=Wang Li , Kaya Hilal Betul , Zhang Ning , Rai Rhitu , Willmann Matthew R. , Carpenter Sara C. D. , Read Andrew C. , Martin Federico , Fei Zhangjun , Leach Jan E. , Martin Gregory B. , Bogdanove Adam J. 

TITLE=Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants

JOURNAL=Frontiers in Genome Editing

VOLUME=3

YEAR=2021

URL=https://www.frontiersin.org/journals/genome-editing/articles/10.3389/fgeed.2021.617553

DOI=10.3389/fgeed.2021.617553

ISSN=2673-3439

ABSTRACT=<p>Prime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of prime editing in plants, a variety of edits in rice and wheat were described, including insertions up to 15 bp. Several studies in rice quickly followed, but none reported a larger insertion. Here, we report easy-to-use vectors for prime editing in dicots as well as monocots, their validation in <italic>Nicotiana benthamiana</italic>, rice, and Arabidopsis, and an insertion of 66 bp that enabled split-GFP fluorescent tagging.</p>