AUTHOR=Nasti Ryan A. , Zinselmeier Matthew H. , Vollbrecht Macy , Maher Michael F. , Voytas Daniel F. 

TITLE=Fast-TrACC: A Rapid Method for Delivering and Testing Gene Editing Reagents in Somatic Plant Cells

JOURNAL=Frontiers in Genome Editing

VOLUME=2

YEAR=2021

URL=https://www.frontiersin.org/journals/genome-editing/articles/10.3389/fgeed.2020.621710

DOI=10.3389/fgeed.2020.621710

ISSN=2673-3439

ABSTRACT=<p>The production of transgenic or gene edited plants requires considerable time and effort. It is of value to know at the onset of a project whether the transgenes or gene editing reagents are functioning as predicted. To test molecular reagents transiently, we implemented an improved, <italic>Agrobacterium tumefaciens</italic>-based co-culture method called Fast-TrACC (Fast Treated Agrobacterium Co-Culture). Fast-TrACC delivers reagents to seedlings, allowing high throughput, and uses a luciferase reporter to monitor and calibrate the efficiency of reagent delivery. We demonstrate the use of Fast-TrACC in multiple solanaceous species and apply the method to test promoter activity and the effectiveness of gene editing reagents.</p>