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CORRECTION article

Front. Genet.
Sec. Genomic Assay Technology
Volume 16 - 2025 | doi: 10.3389/fgene.2025.1553186

Corrigendum: Set of 15 SNP-SNP markers for detection of unbalanced degraded DNA mixtures and noninvasive prenatal paternity testing

Provisionally accepted
Ranran Zhang Ranran Zhang 1Yu Tan Yu Tan 2Li Wang Li Wang 2Hui Jian Hui Jian 1Jing Zhu Jing Zhu 1Yuanyuan Xiao Yuanyuan Xiao 1Mengyu Tan Mengyu Tan 1Jiaming Xue Jiaming Xue 1Fan Yang Fan Yang 2Weibo Liang Weibo Liang 3*
  • 1 Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China
  • 2 Department of Obstetrics and Gynecology, West China Second University Hospital, Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, Sichuan University, Chengdu, China
  • 3 Sichuan University, Chengdu, China

This article is a correction to:

  1. Set of 15 SNP-SNP Markers for Detection of Unbalanced Degraded DNA Mixtures and Noninvasive Prenatal Paternity Testing

    1. Read original article

The final, formatted version of the article will be published soon.

    Unbalanced and degraded mixtures (UDM) are very common in forensic DNA analysis. For example, DNA signals from criminal suspects are masked by a large amount of DNA from victims, or cell-free fetal DNA (cffDNA) in maternal plasma is masked by a high background of maternal DNA. Currently, detecting minor DNA in these mixtures is complex and challenging. We developed a new set of SNP-SNP microhaplotypes with short amplicons, and we successfully genotyped them using the new method of amplification-refractory mutation system PCR (ARMS-PCR) combined with SNaPshot technology based on a capillary electrophoresis (CE) platform. This panel reflects a high polymorphism in the Southwest Chinese Han population and thus has excellent potential for mixture studies. We evaluated the feasibility of this panel for UDM detection and noninvasive prenatal paternity testing (NIPPT). Fifteen SNP-SNPs detected minor DNA of homemade DNA mixtures, with a sensitivity of 0.025–0.05 ng and a specificity of 1:1,000. In addition, the panel successfully genotyped degraded DNA from single and mixed samples. Finally, 15 SNP-SNPs were applied to 26 trios. All samples displayed positive results with at least one marker to detect cffDNA. Besides, all fetal alleles in maternal plasma were confirmed by genotyping fetal genomic DNA from amniocentesis and paternal genomic DNA from peripheral blood. The results indicated that the SNP-SNP strategy based on the CE platform was useful for UDM detection and NIPPT.

    Keywords: SNP-SNP microhaplotype, Amplification-refractory mutation system PCR (ARMS-PCR), snapshot, Unbalanced and degraded mixture (UDM), Cell-free fetal DNA (cfDNA), Noninvasive prenatal paternity testing (NIPPT)

    Received: 06 Jan 2025; Accepted: 06 Jan 2025.

    Copyright: © 2025 Zhang, Tan, Wang, Jian, Zhu, Xiao, Tan, Xue, Yang and Liang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Weibo Liang, Sichuan University, Chengdu, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.