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ORIGINAL RESEARCH article
Front. Genet.
Sec. RNA
Volume 16 - 2025 | doi: 10.3389/fgene.2025.1474223
This article is part of the Research Topic Recent Advancements in RNA-based and targeted Therapeutics View all 5 articles
A comprehensive analysis of microRNA alteration in ApoE(-/-) mice model of white adipose tissue injury induced by chronic intermittent hypoxia
Provisionally accepted- 1 Fujian Medical University, Fuzhou, Fujian Province, China
- 2 The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China
Background: MicroRNAs (miRNAs) represent a series of non-coding small RNAs and are involved in the processes of many diseases. However, the role of microRNA in obstructive sleep apnea (OSA)-induced white adipose tissue (WAT) dysfunction remains to be elaborated. Taking advantage of miRNA sequencing (miRNA-seq), we uncovered the miRNA expression profiles in chronic intermittent hypoxia (CIH)-induced WAT dysfunction mice.We established apolipoprotein deficient (ApoE-/-) mice CIH model and traced differential expressed miRNAs (DEmiRs) through miRNA-seq technology. With the help of Gene Ontology (GO) functional enrichment and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, we revealed the biological functions of these DEmiRs. In addition, RT-qPCR was used for further evaluation of the sequencing data. Ultimately, we designed a CNC network to expound the relationship between miRNA and target genes.In general, 13 miRNAs manifested as upregulated while 18 miRNAs as downregulated in a CIH mouse model of WAT dysfunction. KEGG results indicated that the Lysosome pathway participated in CIH-induced WAT dysfunction. Then, we selected eight miRNAs for RT-qPCR validation. Based on the data, we chose these DEmiRs to construct a miRNA-mRNA network.To sum up, we discovered 31 DEmiRs in the ApoE-/-mice model of CIH. Our finding may play a major role in explaining the pathophysiology mechanisms of WAT dysfunction induced by obstructive sleep apnea.
Keywords: microRNA, chronic intermittent hypoxia, white adipose tissue dysfunction, miRNA sequencing, Bioinformatics analysis
Received: 01 Aug 2024; Accepted: 26 Feb 2025.
Copyright: © 2025 Zhang, Chen, Guo, Ji, Lin and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Qingshi Chen, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China
Dexin Liu, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China
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