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ORIGINAL RESEARCH article

Front. Genet.
Sec. RNA
Volume 16 - 2025 | doi: 10.3389/fgene.2025.1396073

Alteration of chromatin states perturb the transcription regulation of gene during hydronephrosis

Provisionally accepted
Xiao-hui Wang Xiao-hui Wang Shu-Feng Zhang Shu-Feng Zhang *Hai-ying Wu Hai-ying Wu Jian Gao Jian Gao Lin Wang Lin Wang Yao Yin Yao Yin Xuhui Wang Xuhui Wang
  • Henan Provincial People's Hospital, Zhengzhou, Henan Province, China

The final, formatted version of the article will be published soon.

    Gene expression is abnormal in disease compared to normal tissue same as the regulatory elements. Regulatory element binding with transcription factors managed transcription of gene, which usually require chromatin accessible. To reveal potential epigenetic mechanism during hydronephrosis, we first used RNA-seq to finger out the disfunction genes during hydronephrosis, then combined with ATAC-seq, and BS-seq to reveal the related disfunction regulatory elements.Finally, we find that 860 differentially genes and 2429 dynamic chromatin open regions between normal and hydronephrosis tissue. Though, most of disfunction genes and regulatory elements significantly enriched in chronic kidney disease GO term, only small part of regulatory element target genes overlapped with truly disfunction genes. And we also find out an important gene OTUD6B, which overexpression in disease tissue is manipulated by distal regulatory element through chromatin loop, and confirm the importance of epigenetic mechanism in disease.

    Keywords: Hydronephrosis, atac seq, BS-Seq, GO term and KEGG pathways, OTUD6B

    Received: 05 Mar 2024; Accepted: 23 Jan 2025.

    Copyright: © 2025 Wang, Zhang, Wu, Gao, Wang, Yin and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Shu-Feng Zhang, Henan Provincial People's Hospital, Zhengzhou, 450000, Henan Province, China

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