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BRIEF RESEARCH REPORT article

Front. Genet.
Sec. RNA
Volume 15 - 2024 | doi: 10.3389/fgene.2024.1527223

Comprehensive analysis of the circRNA expression profile and circRNA-miRNA-mRNA network in pelvic organ prolapse

Provisionally accepted
Qian Wang Qian Wang Zuoxi He Zuoxi He Lisha Ding Lisha Ding Yuqing Liu Yuqing Liu Xiaoli Zhang Xiaoli Zhang Tao Wang Tao Wang *Xiaoyu Niu Xiaoyu Niu *
  • West China Second University Hospital, Sichuan University, Chengdu, China

The final, formatted version of the article will be published soon.

    Pelvic organ prolapse (POP) is a common gynecological disease caused by pathological defects, lesions, or mechanical weakening of the support structures of the pelvic floor. However, its pathogenesis is unclear. Circular RNAs (circRNAs) are covalently closed, endogenous biomolecules, which are thought to play an important role on skeletal muscle development by regulating gene expression. In this study, five pairs of peripheral blood samples from control and POP groups were used for circRNA sequencing analysis to obtain differential expression profiles. A total of 75 differentially expressed circRNAs (DEcircRNAs) were identified (fold change > 2.0, P < 0.05). Furthermore, RT-qPCR confirmed that the expression levels of two circRNAs (hsa_circ_0067962 and hsa_circ_0057051) were significantly lower in the POP group. The two validated DEcircRNAs were abundantly involved in the collagen catabolic process. The circRNA-miRNA-mRNA network of two DEcircRNAs comprised nine mRNAs, which indicated that hsa_circ_0067962 and hsa_circ_0057051 may be involved in the pathogenesis of POP by regulating these nine mRNAs.

    Keywords: Pelvic Organ Prolapse, circRNA sequencing, Peripheral Blood, Differentially expressed circRNAs, circRNA-miRNA-mRNA network

    Received: 13 Nov 2024; Accepted: 31 Dec 2024.

    Copyright: © 2024 Wang, He, Ding, Liu, Zhang, Wang and Niu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Tao Wang, West China Second University Hospital, Sichuan University, Chengdu, China
    Xiaoyu Niu, West China Second University Hospital, Sichuan University, Chengdu, China

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