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ORIGINAL RESEARCH article

Front. Genet.
Sec. Human and Medical Genomics
Volume 15 - 2024 | doi: 10.3389/fgene.2024.1522809
This article is part of the Research Topic Genetic and molecular research on osteoarthritis and osteoporosis View all articles

Comprehensive analysis of ferroptosis-related genes reveals potential therapeutic targets in osteoporosis patients: a computational analysis and in vitro experiments

Provisionally accepted
Sihui Chen Sihui Chen Yi Jiang Yi Jiang *Guoqing Xie Guoqing Xie *Peng Wu Peng Wu *Jinyu Zhu Jinyu Zhu *
  • First Hospital of Jiaxing, Jiaxing, China

The final, formatted version of the article will be published soon.

    Background: Ferroptosis-related genes have been reported to play important roles in many diseases, but their molecular mechanisms in osteoporosis have not been elucidated.Based on two independent GEO datasets (GSE35956 and GSE35958), and GSE35959 as the validation dataset, we comprehensively elucidated the pathological mechanism of ferroptosis-related genes in osteoporosis by GO analyses, KEGG analyses and a PPI network. Then, We used Western Blot (WB) and Quantitative real-time polymerase chain reaction (qPCR) to verify the expression level of KMT2D, a ferroptosis-related hub gene, in clinical samples.Subsequently, we predicted the upstream miRNA of KMT2D gene and analyzed the mechanism of KMT2D in osteoporosis, the potential prognostic value and its immune invasion of KMT2D in pan-cancer Results: This study identified KMT2D and MYCN, TP63, RELA, SOX2, and CDKN1A as key ferroptosis-related genes in osteoporotic cell aging. The independent dataset validated that the expression level of KMT2D was significantly upregulated in osteoporosis samples.The experimental verification results of qPCR and WB indicate that KMT2D is highly expressed in patients with osteoporosis. Further analysis revealed that the hsa-miR-204-5p-KMT2D axis may play an important role in the aging of osteoporotic cells.The analysis of KMT2D reveals that KMT2D may mainly play a role in the aging of osteoporotic cells through epigenetics and the value in pan-cancer.The study provides a theoretical basis for the treatment of osteoporosis.

    Keywords: KMT2D, Osteoporosis, ferroptosis, Aging, BMSCs

    Received: 05 Nov 2024; Accepted: 18 Dec 2024.

    Copyright: © 2024 Chen, Jiang, Xie, Wu and Zhu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yi Jiang, First Hospital of Jiaxing, Jiaxing, China
    Guoqing Xie, First Hospital of Jiaxing, Jiaxing, China
    Peng Wu, First Hospital of Jiaxing, Jiaxing, China
    Jinyu Zhu, First Hospital of Jiaxing, Jiaxing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.