Skip to main content

ORIGINAL RESEARCH article

Front. Genet.
Sec. Stem Cell Research
Volume 15 - 2024 | doi: 10.3389/fgene.2024.1515778

Differential regulation of apoptosis-related genes during long-term culture and differentiation of canine adipose-derived stem cells -a functional bioinformatical analysis

Provisionally accepted
Maurycy Jankowski Maurycy Jankowski 1,2,3*Katarzyna Stefańska Katarzyna Stefańska 1Michał Suchodolski Michał Suchodolski 3Claudia Dompé Claudia Dompé 4,5Grzegorz Wąsiatycz Grzegorz Wąsiatycz 6Bartosz Kempisty Bartosz Kempisty 6,7,8,9Michał Nowicki Michał Nowicki 2Magdalena Roszak Magdalena Roszak 1
  • 1 Department of Computer Sciences and Statistics, Poznan University of Medical Sciences, Poznan, Poland
  • 2 Department of Histology and Embryology, Faculty of Medicine, Poznan University of Medical Sciences, Poznan, Poland
  • 3 Greater Poland Center of Digital Medicine, Poznan University of Medical Sciences, Poznan, Poland
  • 4 Department of Immunology, Poznan University of Medical Sciences, Poznan, Poland
  • 5 Doctoral School, Poznan University of Medical Sciences, Poznan, Poland
  • 6 Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, Torun, Poland
  • 7 Division of Anatomy, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, Wroclaw, Poland
  • 8 Physiology Graduate Faculty, North Carolina State University, Raleigh, United States
  • 9 Center of Assisted Reproduction, Department of Obstetrics and Gynecology, University Hospital and Masaryk University, Brno, Czechia

The final, formatted version of the article will be published soon.

    Stem cells derived from adipose tissue are gaining popularity in the field of regenerative medicine due to their adaptability and clinical potential. Their rapid growth, ability to differentiate, and easy extraction with minimal complications make adipose-derived stem cells (ADSCs) a promising option for many treatments, particularly those targeting bone-related diseases. This study analyzed gene expression in canine ADSCs subjected to long-term culture and osteogenic differentiation. ADSCs were isolated from discarded surgical waste and cultured for 14 days with and without differentiation media to assess osteogenic changes. RNA sequencing (RNA-seq) and bioinformatical analysis were performed to obtain comprehensive transcriptomic data. A total of 17793 genes were detected and GO enrichment analysis was performed on the differentially expressed genes to identify significantly up-and downregulated Biological Process (BP) GO terms across each comparison. The upregulation of apoptosis-regulating genes and genes related to circulatory system development suggest an induction of these processes, while the downregulation of neurogenesis and gliogenesis genes points to reciprocal regulation during osteogenic differentiation of canine ADSCs. These findings underscore the potential of ADSCs in bone regeneration and offer valuable insights for advancing tissue engineering, however further studies, including proteomic analyses, are needed to confirm these patterns and their biological significance.

    Keywords: Adipose Tissue, Stem Cells, Transcriptomics, Gene Expression, gene ontology, Apoptosis

    Received: 24 Oct 2024; Accepted: 11 Dec 2024.

    Copyright: © 2024 Jankowski, Stefańska, Suchodolski, Dompé, Wąsiatycz, Kempisty, Nowicki and Roszak. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Maurycy Jankowski, Department of Computer Sciences and Statistics, Poznan University of Medical Sciences, Poznan, Poland

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.