Pengchao Gao
Jiancai Xiao
Yan Zhang
*
Tie-Gui Nan
*The final, formatted version of the article will be published soon.
ORIGINAL RESEARCH article
Front. Genet.
Sec. Genomics of Plants and the Phytoecosystem
Volume 15 - 2024 |
doi: 10.3389/fgene.2024.1442277
This article is part of the Research Topic Multi-omics and molecular biology studies on abiotic stress in crops View all 3 articles
Genome-Wide Identification of Glycyrrhiza uralensis Fisch. MAPK Gene Family and Exp ression Analysis under Salt Stress Relieved by Bacillus subtilis
Provisionally accepted- China Academy of Chinese Medical Sciences, Beijing, China
Research on Glycyrrhiza uralensis, a nonhalophyte that thrives in saline-alkaline soil and a traditional Chinese medicinal component, is focused on improving its ability to tolerate salt stress to increase its pro ductivity and preserve its "Dao-di" characteristics. Furthermore, the inoculation of bioagents such as Bacill us subtilis to increase plant responses to abiotic stressors is currently a mainstream strategy. Mitogen-activ ated protein kinase (MAPK), a highly conserved protein kinase, plays a significant role in plant responses to various abiotic stress pathways. This investigation involved the identification of 21 members of the Gu MAPK family from the genome of G. uralensis, with an analysis of their protein conserved domains, gene structures, evolutionary relationships, and phosphorylation sites using bioinformatics tools. Systematic evol utionary analysis of the 21 GuMAPKs classified them into four distinct subgroups, revealing significant dif ferences in gene structure and exon numbers. Collinearity analysis highlighted the crucial role of segmenta l duplication in expanding the GuMAPK gene family, which is particularly evident in G. uralensis and sh ows a close phylogenetic relationship with Arabidopsis thaliana, tomato, and cucumber. Additionally, the i dentification of phosphorylation sites suggests a strong correlation between GuMAPK and various physiolog ical processes, including hormonal responses, stress resistance, and growth and development. Protein interac tion analysis further supported the role of GuMAPK proteins in regulating essential downstream genes. Thr ough examination of transcriptome expression patterns, GuMAPK16-2 emerged as a prospective pivotal reg ulatory factor in the context of salt stress and B. subtilis inoculation, a finding supported by its subcellula r localization within the nucleus. These discoveries offer compelling evidence for the involvement of GuM APK in the salt stress response and for the exploration of the mechanisms underlying B. subtilis' enhance ment of salt tolerance in G. uralensis. Article II.
Keywords: glycyrrhiza uralensis Fish., salt stress, MAPK, Bacillus subtilis, Subcellular localization
Received: 01 Jun 2024; Accepted: 15 Jul 2024.
Copyright: © 2024 Gao, Xiao, Guo, Fan, Zhang and Nan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yan Zhang, China Academy of Chinese Medical Sciences, Beijing, China
Tie-Gui Nan, China Academy of Chinese Medical Sciences, Beijing, China
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