AUTHOR=Guan Xuefeng , Fan Yuan , Six Rani , Benedetti Camilla , Raes Annelies , Fernandez Montoro Andrea , Cui Xiaole , Azari Dolatabad Nima , Van Soom Ann , Pavani Krishna Chaitanya , Peelman Luc TITLE=Bta-miR-665 improves bovine blastocyst development through its influence on microtubule dynamics and apoptosis JOURNAL=Frontiers in Genetics VOLUME=15 YEAR=2024 URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2024.1437695 DOI=10.3389/fgene.2024.1437695 ISSN=1664-8021 ABSTRACT=
Extracellular vesicles (EVs) contain microRNAs (miRNAs), which are important regulators of embryonic development. Nevertheless, little is known about the precise molecular processes controlling blastocyst development and quality. In a previous study, we identified bta-miR-665 as one of the miRNAs more abundantly present in extracellular vesicles of embryo-conditioned culture media of blastocysts compared to degenerate ones. Here, we investigated the effect and regulatory roles of bta-miR-665 in blastocyst development by supplementation of bta-miR-665 mimics or inhibitors to the culture media. Supplementation of bta-miR-665 mimics improved cleavage and blastocyst rate (