Skip to main content

ORIGINAL RESEARCH article

Front. Genet.
Sec. Cancer Genetics and Oncogenomics
Volume 15 - 2024 | doi: 10.3389/fgene.2024.1402856

Construction of circRNA-mediated ceRNA network and immunoassay for investigating pathogenesis of COPD

Provisionally accepted
Ting Yang Ting Yang 1Wenya Xu Wenya Xu 2Jie Zhao Jie Zhao 2Jie Chen Jie Chen 1Siguang Li Siguang Li 1Lingsang Lin Lingsang Lin 1Yi Zhong Yi Zhong 1Zehua Yang Zehua Yang 2Tian Xie Tian Xie 2Yipeng Ding Yipeng Ding 1*
  • 1 Department of General Practice, Hainan General Hospital, Haikou, Hainan Province, China
  • 2 Department of Pulmonary and Critical Care Medicine, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, China

The final, formatted version of the article will be published soon.

    The chronic respiratory condition known as chronic obstructive pulmonary disease (COPD) was one of the main causes of death and disability worldwide. This study aimed to explore and elucidate new targets and molecular mechanisms of COPD by constructing competitive endogenous RNA (ceRNA) networks.Methods: GSE38974 and GSE106986 were used to select DEGs in COPD samples and normal samples. Cytoscape software was used to construct and present protein-protein interaction (PPI) network, mRNA-miRNA co-expression network and ceRNA network. The CIBERSORT algorithm and the Lasso model were used to screen the immune infiltrating cells and hub genes associated with COPD, and the correlation between them was analyzed. COPD cell models were constructed in vitro and the expression level of ceRNA network factors mediated by hub gene was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR).Results: In this study, 852 differentially expressed genes were screened in the GSE38974 dataset, including 439 up-regulated genes and 413 down-regulated genes.Gene clustering analysis of PPI network results was performed using the Minimum Common Tumor Data Element (MCODE) in Cytoscape, and seven hub genes were screened using five algorithms in cytoHubba. CCL20 was verified as an important hub gene based on mRNA-miRNA co-expression network, GSE106986 database validation and the analysis of ROC curve results. Finally, we successfully constructed the circDTL-hsa-miR-330-3p-CCL20 network by Cytoscape. Immune infiltration analysis suggested that CCL20 can co-regulate immune cell migration and infiltration through chemokines CCL7 and CXCL3. In vitro experiments, the expression of circDTL and CCL20 was increased, while the expression of hsa-miR-330-3p was decreased in the COPD cell model.By constructing the circDTL-hsa-miR-330-3p-CCL20 network, this study contributes to a better understanding of the molecular mechanism of COPD development, which also provides important clues for the development of new therapeutic strategies and drug targets.

    Keywords: chronic obstructive pulmonary disease, circDTL, hsa-miR-330-3p, CCL20, competitive endogenous RNA

    Received: 18 Mar 2024; Accepted: 12 Aug 2024.

    Copyright: © 2024 Yang, Xu, Zhao, Chen, Li, Lin, Zhong, Yang, Xie and Ding. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Yipeng Ding, Department of General Practice, Hainan General Hospital, Haikou, 570311, Hainan Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.