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ORIGINAL RESEARCH article

Front. Genet.
Sec. RNA
Volume 15 - 2024 | doi: 10.3389/fgene.2024.1365232

Identification and validation of ferroptosis related markers in erythrocyte differentiation of umbilical cord blood-derived CD34+ cell by bioinformatic analysis

Provisionally accepted
Qian Liu Qian Liu 1Ze Lin Ze Lin 2Minghui Yue Minghui Yue 2Jianbo Wu Jianbo Wu 1Lei li Lei li 1Daqi Huang Daqi Huang 1Yipeng Fang Yipeng Fang 3Xin Zhang Xin Zhang 2*Tao Hao Tao Hao 1*
  • 1 Binzhou Medical University Hospital, Binzhou, Shandong Province, China
  • 2 Shantou University Medical College, Shantou, China
  • 3 Tianjin Medical University General Hospital, Tianjin, China

The final, formatted version of the article will be published soon.

    Ferroptosis has been observed to play an important role during erythrocyte differentiation (ED). However, the biological gene markers and ferroptosis mechanisms in ED remain unknown. We downloaded the datasets of ED in human umbilical cord blood-derived CD34+ cells from the Gene Expression Omnibus database. Using median differentiation time, the sample was categorized into long and short groups. The differentially expressed ferroptosis-related genes (DE-FRGs) were screened using differential expression analysis. The enrichment analyses and a protein-protein interaction (PPI) network were conducted. To predict the ED stage, a logistic regression model was constructed using the least absolute shrinkage and selection operator (LASSO). Overall, 22 DE-FRGs were identified. Ferroptosis-related pathways were enriched using Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes. Gene Set Enrichment Analysis and Gene Set Variation Analysis revealed the primary involvement of DE-FRGs in JAK-STAT, MAPK, PI3K-AKT-mTORC1, WNT, and NOTCH signaling pathways. Ten-hub DE-FRGs were obtained using PPI analysis. Furthermore, we constructed mRNA-microRNA (miRNA) and mRNA-transcription factor networks. Immune cell infiltration levels differed significantly during ED. LASSO regression analysis established a signature using six DE-FRGs (ATF3, CDH2, CHAC1, DDR2, DPP4, and GDF15) related to the ED stage. Bioinformatic analyses identified ferroptosis-associated genes during ED, which were further validated. Overall, we identified ferroptosis-related genes to predict their correlations in ED. Exploring the underlying mechanisms of ferroptosis may help us better understand pathophysiological changes in ED and provide new evidence for clinical transformation.

    Keywords: ferroptosis, erythrocyte differentiation, Human umbilical cord blood-derived CD34+ cell, Immunocyte infiltration, Bioinformatic analysis

    Received: 04 Jan 2024; Accepted: 09 Jul 2024.

    Copyright: © 2024 Liu, Lin, Yue, Wu, li, Huang, Fang, Zhang and Hao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xin Zhang, Shantou University Medical College, Shantou, China
    Tao Hao, Binzhou Medical University Hospital, Binzhou, 256603, Shandong Province, China

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