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ORIGINAL RESEARCH article

Front. Genet.
Sec. Genetics of Common and Rare Diseases
Volume 15 - 2024 | doi: 10.3389/fgene.2024.1335093

Authentication and validation of key genes in the treatment of atopic dermatitis with Runfuzhiyang powder: A combined RNA-seq, bioinformatics analysis, and experimental research

Provisionally accepted
Yan Lin Yan Lin 1*Guangyi Xiong Guangyi Xiong 2*Xiansong Xia Xiansong Xia 3*Zhiping Yin Zhiping Yin 1*Zou Xuhui Zou Xuhui 1Xu Zhang Xu Zhang 1*Chenghao Zhang Chenghao Zhang 1*Jianzhou Ye Jianzhou Ye 1*
  • 1 The No.1 affiliated hospital of Yunnan university of CM, Kunming, China
  • 2 Basic medical science school, Yunnan university of CM, Kunming, Yunnan Province, China
  • 3 Teaching Affairs department, Yunnan university of CM, Kunming, Yunnan Province, China

The final, formatted version of the article will be published soon.

    Atopic dermatitis (AD) is a prevalent chronic inflammatory skin disease characterized by frequent relapses. This study aims to mineidentify key biomarkers toand explore the potential molecular mechanism formechanisms underlying AD incidence. It also investigates, as well as to investigate the therapeutic mechanismeffects of Runfuzhiyang powder on AD. Differentially expressed AD-related genes were acquiredidentified by intersecting key module genes related to thefrom control group, AD group, and treatment group, which were groups and screened bythrough weighted correlation network analysis. Four AD-related biomarkers (-Ddit4, Sbf2, Senp8, and Zfp777) -were identified through thepinpointed using the least absolute shrinkage and selection operator regression analysis. The receiverReceiver operating characteristic curves revealeddemonstrated that these four biomarkers can be employed toeffectively distinguish between the AD group and control group andgroups, as well as between the AD group and treatment groupgroups. Gene set enrichment analysis revealed that these four biomarkers are linked these biomarkers to the pathways ofinvolving E2F targets, KRAS signaling upregulation, and inflammatory response. In additionresponses. Additionally, Ddit4, Sbf2, and Zfp777 were significantly positively correlatedshowed significant positive correlations with M0 macrophages and significantly negatively correlatedsignificant negative correlations with resting NK cells, while it wasSenp8 exhibited the opposite for Senp8. Finallypattern. Furthermore, a transcription factor-mRNA-long noncoding RNA network includingcomprising 200 nodes and 592 edges was generated, andconstructed, predicting 20 drugs targeting SENP8 were predicted. We believe that our study results. These findings may help design facilitate the development of novel strategies for the targeted diagnosis and treatment of AD.

    Keywords: atopic dermatitis, Runfuzhiyang powder, diagnosis, biomarkers, Immune infiltration, TF-mRNA-lncRNA network

    Received: 08 Nov 2023; Accepted: 08 Jul 2024.

    Copyright: © 2024 Lin, Xiong, Xia, Yin, Xuhui, Zhang, Zhang and Ye. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yan Lin, The No.1 affiliated hospital of Yunnan university of CM, Kunming, China
    Guangyi Xiong, Basic medical science school, Yunnan university of CM, Kunming, Yunnan Province, China
    Xiansong Xia, Teaching Affairs department, Yunnan university of CM, Kunming, Yunnan Province, China
    Zhiping Yin, The No.1 affiliated hospital of Yunnan university of CM, Kunming, China
    Xu Zhang, The No.1 affiliated hospital of Yunnan university of CM, Kunming, China
    Chenghao Zhang, The No.1 affiliated hospital of Yunnan university of CM, Kunming, China
    Jianzhou Ye, The No.1 affiliated hospital of Yunnan university of CM, Kunming, China

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