AUTHOR=Hashemi Kimia Sadat , Aliabadi Mohadese Koohi , Mehrara Arian , Talebi Elham , Hemmati Ali Akbar , Rezaeiye Radin Dabbagh , Ghanbary Mohammad Javad , Motealleh Maryam , Dayeri Behnaz , Alashti Shayan Khalili 

TITLE=A meta-analysis of microarray datasets to identify biological regulatory networks in Alzheimer’s disease

JOURNAL=Frontiers in Genetics

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2023.1225196

DOI=10.3389/fgene.2023.1225196

ISSN=1664-8021

ABSTRACT=<p><bold>Background:</bold> Alzheimer’s Disease (AD) is an age-related progressive neurodegenerative disorder characterized by mental deterioration, memory deficit, and multiple cognitive abnormalities, with an overall prevalence of ∼2% among industrialized countries. Although a proper diagnosis is not yet available, identification of miRNAs and mRNAs could offer valuable insights into the molecular pathways underlying AD’s prognosis.</p><p><bold>Method:</bold> This study aims to utilize microarray bioinformatic analysis to identify potential biomarkers of AD, by analyzing six microarray datasets (GSE4757, GSE5281, GSE16759, GSE28146, GSE12685, and GSE1297) of AD patients, and control groups. Furthermore, this study conducted gene ontology, pathways analysis, and protein-protein interaction network to reveal major pathways linked to probable biological events. The datasets were meta-analyzed using bioinformatics tools, to identify significant differentially expressed genes (DEGs) and hub genes and their targeted miRNAs’.</p><p><bold>Results:</bold> According to the findings, <italic>CXCR4</italic>, <italic>TGFB1</italic>, <italic>ITGB1</italic>, <italic>MYH11</italic>, and <italic>SELE</italic> genes were identified as hub genes in this study<italic>.</italic> The analysis of DEGs using GO (gene ontology) revealed that these genes were significantly enriched in actin cytoskeleton regulation, ECM-receptor interaction, and hypertrophic cardiomyopathy. Eventually, <italic>hsa-mir-122-5p, hsa-mir-106a-5p, hsa-mir-27a-3p, hsa-mir16-5p, hsa-mir-145-5p, hsa-mir-12-5p, hsa-mir-128-3p, hsa-mir 3200-3p, hsa-mir-103a-3p</italic>, and <italic>hsa-mir-9-3p</italic> exhibited significant interactions with most of the hub genes.</p><p><bold>Conclusion:</bold> Overall, these genes can be considered as pivotal biomarkers for diagnosing the pathogenesis and molecular functions of AD.</p>