AUTHOR=Wang Qingchun , Bao Haiying , Li Zhijun 

TITLE=Genomic comparison between two Inonotus hispidus strains isolated from growing in different tree species

JOURNAL=Frontiers in Genetics

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2023.1221491

DOI=10.3389/fgene.2023.1221491

ISSN=1664-8021

ABSTRACT=<p><italic>Inonotus hispidus</italic> mainly growing in broad-leaved trees, including <italic>Morus alba</italic>, <italic>Fraxinus mandshurica</italic>, and <italic>Ulmus macrocarpa</italic> etc. The fruiting body of <italic>I. hispidus</italic> growing in <italic>M. alba</italic> (hereafter as MA) is used as a traditional Chinese medicine “<italic>Sanghuang</italic>”. However, differences between the genetic material basis of <italic>I. hispidus</italic> growing in other tree species have not been reported. Therefore, in this paper, the genomic comparison between MA and <italic>I. hispidus</italic> growing in <italic>F. mandshurica</italic> (hereafter as FM) were studied. The whole genome of MA monokaryon was sequenced by Illumina combined with Pac Bio platform. Next, genome assembly, genome component prediction and genome functional annotation were performed. Comparative genomics analysis was performed between FM monokaryon and MA monokaryon, using MA as the reference. The results showed that, MA had 24 contigs with a N50 length of 2.6 Mb. Specifically, 5,342, 6,564, 1,595, 383 and 123 genes were annotated from GO, KEGG, KOG, CAZymes and CYP450, respectively. Moreover, comparative genomics showed that, the coding genes and total number of genes annotated in different databases of FM were higher than that of MA. This study provides a foundation for the medicinal application of FM as MA from the perspective of genetic composition.</p>