AUTHOR=Liu Yu , Huang Yuning , Li Zhao , Feng Ming , Ge Weide , Zhong Chao , Xue Renfeng 

TITLE=Genome-wide identification of the TGA genes in common bean (Phaseolus vulgaris) and revealing their functions in response to Fusarium oxysporum f. sp. phaseoli infection

JOURNAL=Frontiers in Genetics

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2023.1137634

DOI=10.3389/fgene.2023.1137634

ISSN=1664-8021

ABSTRACT=<p>Fusarium wilt, which affects common bean all across the world, is caused by <italic>Fusarium oxysporum</italic> f. sp. <italic>Phaseoli</italic> (<italic>Fop</italic>). It is necessary to have functional genes in response to <italic>Fop</italic> infection because they might be used to manage disease. As a crucial regulator, <italic>TGA</italic>-binding transcription factor (<italic>TGA</italic>) is engaged in the defense mechanism of plants against pathogens. The role of <italic>TGA</italic> regulators in common bean in response to <italic>Fop</italic> infection, however, has not been documented. Hence, we performed genome-wide identified and characterized eight <italic>TGA</italic> genes in common bean. In this study, eight <italic>PvTGA</italic> genes were distributed on six chromosomes and classified into four subgroups. The <italic>PvTGA</italic> genes have the same conserved bZIP and DOG1 domains, but there are specific sequence structures in different <italic>PvTGAs</italic>. Phylogenetic and synteny analysis explained that <italic>PvTGA</italic> gene has a close genetic relationship with legume <italic>TGAs</italic> and that <italic>PvTGA03</italic> and <italic>PvTGA05</italic> may play an important role in evolution. Transcriptome data explained that expression levels of <italic>PvTGA</italic> genes showed diversity in different tissues. After <italic>Fop</italic> inoculation, the expression levels of <italic>PvTGA0</italic>3 and <italic>PvTGA07</italic> were significantly different between resistant and susceptible genotypes. Under SA treatment, the expression levels of <italic>PvTGA03</italic>, <italic>PvTGA04</italic>, <italic>PvTGA06</italic>, <italic>PvTGA07</italic> and <italic>PvTGA08</italic> were significantly different. These results imply that <italic>PvTGA03</italic> and <italic>PvTGA07</italic> play key roles in SA-mediated resistance to Fusarium wilt. Together, these findings advance knowledge of the <italic>PvTGA</italic> gene family in common bean and will help future studies aimed at reducing Fusarium wilt.</p>