AUTHOR=Wang Zhenling , Huang Changzhi , Wu Jingyu , Zhang Hongqiang , Shao Yu , Fu Zan 

TITLE=Analysis of the Prognostic Significance and Immune Infiltration of the Amino Acid Metabolism-Related Genes in Colon Adenocarcinoma

JOURNAL=Frontiers in Genetics

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.951461

DOI=10.3389/fgene.2022.951461

ISSN=1664-8021

ABSTRACT=Amino acid metabolization is verified to take part in the progression of cancer. However, genes related to amino acid metabolism have not been identified in colon adenocarcinoma (COAD). A systematic prognostic model of COAD becomes a pressing need. RIMKLB, ASPG, TH, MTAP, AZIN2, PSMB2, HDC, ACMSD, and PSMA8 were identified to construct a risk model. Kaplan-Meier (K-M) analyses demonstrated that the high-risk group achieved a poor prognosis. Area under the respective ROC (AUC) values indicated the robustness of the model. Cox regression reduced clinically characters to age, T, stage, and risk score. A nomogram was shaped to correspond with achieved clinicopathology features. A higher tumor mutation burden was observed in the high-risk group. However, the low-risk group had a stronger immune infiltration. Seven molecular subtypes were found by consensus cluster. 22 hub genes were identified related to ESTIMATE score using WGCNA. In brief, our research constructed a stable prognostic model related to amino acid metabolism in COAD, revealing its connection to the immune microenvironment. The model guided the outcome of COAD and the direction of immunotherapy.