AUTHOR=Zhang Chi , Zhang Kun , Peng Ying , Zhou Jianshe , Liu Yifan , Liu Bingjian 

TITLE=Novel Gene Rearrangement in the Mitochondrial Genome of Three Garra and Insights Into the Phylogenetic Relationships of Labeoninae

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.922634

DOI=10.3389/fgene.2022.922634

ISSN=1664-8021

ABSTRACT=<p>Complete mitochondrial genomes (mitogenomes) can provide valuable information for phylogenetic relationships, gene rearrangement, and molecular evolution. Here, we report the mitochondrial whole genomes of three <italic>Garra</italic> species and explore the mechanisms of rearrangements that occur in their mitochondrial genomes. The lengths of the mitogenomes’ sequences of <italic>Garra dengba</italic>, <italic>Garra tibetana</italic>, and <italic>Garra yajiangensis</italic> were 16,876, 16,861, and 16,835, respectively. They contained 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNA genes, and two identical control regions (CRs). The mitochondrial genomes of three <italic>Garra</italic> species were rearranged compared to other fish mitochondrial genomes. The <italic>tRNA-Thr</italic>, <italic>tRNA-Pro</italic> and CR (<italic>T-P-CR</italic>) genes undergo replication followed by random loss of the <italic>tRNA-Thr</italic> and <italic>tRNA-Pro</italic> genes to form tRNA-Thr, CR1, tRNA-Pro and CR2 (<italic>T-CR-P-CR</italic>). Tandem duplication and random loss best explain this mitochondrial gene rearrangement. These results provide a foundation for future characterization of the mitochondrial gene arrangement of <italic>Labeoninae</italic> and further phylogenetic studies.</p>