AUTHOR=Tian Yuanye , Shao Qi , Gu Jiahong , Tang Yi , Bie Miaomiao , Zhou Yangyifan , Cheng Chunan , Liang Yi , Zhang Qian , Kang Feiwu 

TITLE=LncRNA-mRNA Expression Profiles of Osteoclast After Conditional Knockout HIF-1α

JOURNAL=Frontiers in Genetics

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.909095

DOI=10.3389/fgene.2022.909095

ISSN=1664-8021

ABSTRACT=<p><bold>Background:</bold> Osteoclasts, which are multinucleated cells formed by monocyte fusion, play a key role in bone resorption. Hypoxia-inducible factor (HIF)-1α is vital for the development of osteoclasts in hypoxic environments and during bone resorption. However, additional research is required to further study the HIF-1α-dependent regulation of osteoclast differentiation at the genetic level.</p><p><bold>Methods:</bold> In our study, RNA sequencing (RNA-seq) was used to identify the expression profiles of long noncoding RNAs (lncRNAs) and mRNAs in conditional HIF-1α-knockout osteoclasts.</p><p><bold>Results:</bold> A total of 1,320 mRNAs and 95 lncRNAs were differentially expressed. The expression of lncRNAs MSTRG.7566.12 and MSTRG.31769.2 were strongly negatively correlated with that of Mmp9, Ctsk, etc.</p><p><bold>Conclusion:</bold> Our research provides a basis for further understanding the role of mRNAs and lncRNAs in conditional HIF-1α-knockout osteoclasts, and many of these molecules may be potential targets for treating bone diseases related to HIF-1α.</p>