AUTHOR=Zheng Songyuan , Liu Dongyan , Wang Feifei , Jin Youyan , Zhao Siqiao , Sun Siyu , Wang Sheng 

TITLE=ABCA12 Promotes Proliferation and Migration and Inhibits Apoptosis of Pancreatic Cancer Cells Through the AKT Signaling Pathway

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.906326

DOI=10.3389/fgene.2022.906326

ISSN=1664-8021

ABSTRACT=<p><bold>Background:</bold> As a malignant tumor, pancreatic cancer is difficult to detect in its early stage. Pancreatic cancer progresses rapidly and has a short survival time. Most cases have metastasized to distant organs before diagnosis. The mechanism of induction of pancreatic cancer is not fully understood.</p><p><bold>Methods:</bold> In this study, bioinformatics predicted ATP binding cassette subfamily A member 12 (<italic>ABCA12</italic>) expression in pancreatic tissues and performed survival analysis, risk assessment, and enrichment analysis. The expression of <italic>ABCA12</italic> in 30 pairs of clinical samples was detected by immunohistochemistry and we analyzed its correlation with clinical information. Both reverse transcription polymerase chain reaction (RT–PCR) and western blot analysis were used to detect mRNA and protein expression in cell lines. Two different siRNAs and SW1990 cell line were used to construct pancreatic cancer cell models with <italic>ABCA12</italic> knockdown. Cell viability was evaluated by cell counting kit-8 (CCK-8) and EdU proliferation assays. Wound healing assays and Transwell assays were used to measure the ability of cell migration and invasion. Flow cytometry was used to investigate the effect of <italic>ABCA12</italic> on the proliferation cycle and apoptosis of pancreatic cancer. Western blot analysis detected changes in apoptosis, migration, and other pathway proteins in SW1990 cells after transfection.</p><p><bold>Results:</bold><italic>ABCA12</italic> is highly expressed in pancreatic cancer tissues and cells. After <italic>ABCA12</italic> was knocked down, the proliferation, invasion, and migration of SW1990 cells were significantly reduced, and apoptosis was increased. The changes in pathway proteins suggested that <italic>ABCA12</italic> may regulate the progression of pancreatic cancer through the AKT pathway.</p><p><bold>Conclusion:</bold> We found that <italic>ABCA12</italic> is differentially expressed in pancreatic tissues and cells. <italic>ABCA12</italic> can also affect the biological behavior of pancreatic cancer cells effectively, which may serve as a new target for pancreatic cancer diagnosis and treatment.</p>