AUTHOR=Wang Lingyan , Lv Qingqing , Guo Jiayi , Wang Jianwei , Pan Jing 

TITLE=Transcriptome Profiling and Network Analysis Provide Insights Into the Pathogenesis of Vulvar Lichen Sclerosus

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.905450

DOI=10.3389/fgene.2022.905450

ISSN=1664-8021

ABSTRACT=<p>Vulvar lichen sclerosus (VLS) is a chronic inflammatory dermatosis that affects female anogenital skin. Although VLS is considered a T cell-mediated autoimmune disease, the diagnosis criteria, molecular mechanism, and universally accepted therapies for this disease remain largely unresolved. To explore disease pathogenesis and potential biomarkers, we performed an RNA-Seq-based transcriptome analysis to profile the gene expression of VLS lesions. Differentially expressed gene (DEG) analysis revealed profound changes in expressions of coding genes, microRNAs, and long non-coding RNAs. Pathway and network analysis suggested that T cell activation-associated genes, including <italic>CD3G</italic>, <italic>CD3D</italic>, <italic>CD8B</italic>, <italic>LAT</italic>, <italic>LCK</italic>, <italic>ZAP70</italic>, <italic>CCR5</italic>, <italic>CXCR3</italic>, <italic>CXCL9</italic>, <italic>CXCL10</italic>, and <italic>CXCL11</italic>, were highly expressed in VLS, while <italic>NR4A</italic> family genes (<italic>NR4A1</italic>, <italic>NR4A2</italic>, <italic>NR4A3</italic>), whose coding products inhibit T cell activity, were significantly downregulated, suggesting heightened T cell response in VLS. Neutrophil chemoattractant genes <italic>CXCL1</italic>, <italic>CXCL2</italic>, <italic>CXCL3</italic>, <italic>CXCL8</italic>, and their cognate receptor <italic>CXCR2</italic> were downregulated, suggesting dampened neutrophil activity. We also found the downregulation of genes involved in cell cycle progression, including cyclins (<italic>CCNB1</italic>, <italic>CCNB2</italic>, <italic>CCNL1</italic>, <italic>CCNE1</italic>, and <italic>CCNK</italic>) and centrosome factors (<italic>CENPA</italic>, <italic>CENPE</italic>, <italic>CENPF</italic>, and <italic>CENPN</italic>), while microRNA-203a and let-7, microRNAs known to inhibit cell growth, were found to be upregulated. These data collectively indicate that cell proliferation in VLS is compromised. In sum, these findings comprehensively deciphered key regulatory genes and networks in VLS, which could further our understanding of disease mechanisms and point toward therapeutic strategies.</p>