AUTHOR=Tehseen Muhammad Massub , Tonk Fatma Aykut , Tosun Muzaffer , Randhawa Harpinder Singh , Kurtulus Ezgi , Ozseven Izzet , Akin Behyan , Nur Zulfuagaoglu Ozge , Nazari Kumarse 

TITLE=QTL Mapping of Adult Plant Resistance to Stripe Rust in a Doubled Haploid Wheat Population

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.900558

DOI=10.3389/fgene.2022.900558

ISSN=1664-8021

ABSTRACT=<p>Stripe rust caused by <italic>Puccinia striiformis Westend.</italic> f. sp. <italic>tritici</italic>. is a major bread wheat disease worldwide with yield losses of up to 100% under severe disease pressure. The deployment of resistant cultivars with adult plant resistance to the disease provides a long-term solution to stripe rust of wheat. An advanced line from the International Winter Wheat Improvement Program (IWWIP) 130675 (Avd/Vee#1//1-27-6275/Cf 1770/3/MV171-C-17466) showed a high level of adult plant resistance to stripe rust in the field. To identify the adult plant resistance genes in this elite line, a mapping population of 190 doubled haploid (DH) lines was developed from a cross between line 130675 and the universal stripe rust-susceptible variety Avocet S. The DH population was evaluated at precision wheat stripe rust phenotyping platform, in Izmir during 2019, 2020, and 2021 cropping seasons under artificial inoculations. Composite interval mapping (CIM) identified two stable <italic>QTLs QYr.rcrrc-3B.1</italic>, and <italic>QYr.rcrrc-3B.2</italic>, which were detected in multiple years. In addition to these two <italic>QTLs</italic>, five more <italic>QTLs</italic>, <italic>QYr.rcrrc-1B</italic>, <italic>QYr.rcrrc-2A</italic>, <italic>QYr.rcrrc-3A</italic>, <italic>QYr.rcrrc-5A</italic>, and <italic>QYr.rcrrc-7D</italic>, were identified, which were specific to the cropping year (environment). All <italic>QTLs</italic> were derived from the resistant parent, except <italic>QYr.rcrrc-3A</italic>. The significant QTLs explained 3.4–20.6% of the phenotypic variance. SNP markers flanking the <italic>QTL</italic> regions can be amenable to marker-assisted selection. The best DH lines with high yield, end-use quality, and stripe rust resistance can be used for further selection for improved germplasm. SNP markers flanking the QTL regions can aid in identifying such lines.</p>