AUTHOR=Wei Qiuhui , Liu Yuyang , Lan Kaer , Wei Xin , Hu Tianyuan , Chen Rong , Zhao Shujuan , Yin Xiaopu , Xie Tian 

TITLE=Identification and Analysis of MYB Gene Family for Discovering Potential Regulators Responding to Abiotic Stresses in Curcuma wenyujin

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.894928

DOI=10.3389/fgene.2022.894928

ISSN=1664-8021

ABSTRACT=<p><italic>MYB</italic> superfamily is one of the most abundant families in plants, and plays critical role in plant growth, development, metabolism regulation, and stress response. <italic>Curcuma wenyujin</italic> is the main source plant of three traditional Chinese medicines, which are widely used in clinical treatment due to its diverse pharmacological activities. In present study, 88 <italic>CwMYBs</italic> were identified and analyzed in <italic>C. wenyujin</italic>, including 43 <italic>MYB</italic>-related genes, 42 <italic>R2R3-MYB</italic> genes, two <italic>3R-MYB</italic> genes, and one <italic>4R-MYB</italic> gene. Forty-three MYB-related proteins were classified into several types based on conserved domains and specific motifs, including CCA1-like type, R-R type, Myb-CC type, GARP-like type, and TBR-like type. The analysis of motifs in MYB DBD and no-MYB regions revealed the relevance of protein structure and function. Comparative phylogeny analysis divided 42 R2R3-MYB proteins into 19 subgroups and provided a reference for understanding the functions of some CwMYBs based on orthologs of previously characterized MYBs. Expression profile analysis of <italic>CwMYB</italic> genes revealed the differentially expressed genes responding to various abiotic stresses. Four candidate <italic>MYB</italic> genes were identified by combining the results of phylogeny analysis and expression analysis. <italic>CwMYB10</italic>, <italic>CwMYB18</italic>, <italic>CwMYB39</italic>, and <italic>CwMYB41</italic> were significantly induced by cold, NaCl, and MeJA stress treatments. <italic>CwMYB18</italic> and <italic>CwMYB41</italic> were proved as regulators with activity of transcriptional activation, whereas <italic>CwMYB39</italic> and <italic>CwMYB10</italic> were not. They may participate in the response to abiotic stresses through different mechanisms in <italic>C. wenyujin</italic>. This study was the first step toward understanding the <italic>CwMYB</italic> family and the response to abiotic stresses in <italic>C. wenyujin</italic>.</p>