AUTHOR=Li Jingru , Li Chaozhong , Zhao Yulan , Wu Xinyu , Yu Shuai , Sun Guihu , Ding Peng , Lu Si , Zhang Lijiao , Yang Ping , Peng Yunzhu , Fu Jingyun , Wang Luqiao 

TITLE=Integrated bioinformatics analysis for novel miRNAs markers and ceRNA network in diabetic retinopathy

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.874885

DOI=10.3389/fgene.2022.874885

ISSN=1664-8021

ABSTRACT=<p>In order to seek a more outstanding diagnosis and treatment of diabetic retinopathy (DR), we predicted the miRNA biomarkers of DR and explored the pathological mechanism of DR through bioinformatics analysis.</p><p><bold>Method:</bold> Based on public omics data and databases, we investigated ncRNA (non-coding RNA) functions based on the ceRNA hypothesis.</p><p><bold>Result:</bold> Among differentially expressed miRNAs (DE-miRNAs), hsa-miR-1179, -4797-3p and -665 may be diagnosis biomarkers of DR. Functional enrichment analysis revealed differentially expressed mRNAs (DE-mRNAs) enriched in mitochondrial transport, cellular respiration and energy derivation. 18 tissue/organ-specific expressed genes, 10 hub genes and gene cluster modules were identified. The ceRNA networks lncRNA FBXL19-AS1/miR-378f/MRPL39 and lncRNA UBL7-AS1/miR-378f/MRPL39 might be potential RNA regulatory pathways in DR.</p><p><bold>Conclusion:</bold> Differentially expressed hsa-miR-1179, -4797-3p and -665 can be used as powerful markers for DR diagnosis, and the ceRNA network: lncRNA FBXL19-AS1/UBL7-AS1-miR-378f-MRPL39 may represent an important regulatory role in DR progression.</p>