AUTHOR=Kaur Gurwinder , Yadav Inderjit Singh , Bhatia Dharminder , Vikal Yogesh , Neelam Kumari , Dhillon Narpinderjeet Kaur , Praba Umesh Preethi , Mangat Gurjit Singh , Singh Kuldeep 

TITLE=BSA-seq Identifies a Major Locus on Chromosome 6 for Root-Knot Nematode (Meloidogyne graminicola) Resistance From Oryza glaberrima

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.871833

DOI=10.3389/fgene.2022.871833

ISSN=1664-8021

ABSTRACT=<p>Root-knot nematode (<italic>Meloidogyne graminicola</italic>) is one of the emerging threats to rice production worldwide that causes substantial yield reductions. There is a progressive shift of the cropping system from traditional transplanting to direct-seeded water-saving rice production that favored the development of <italic>M. graminicola</italic>. Scouting and deploying new resistance genes is an economical approach to managing the root-knot nematodes. Here, we report that the inheritance of root-knot nematode resistance in <italic>Oryza glaberrima</italic> acc. IRGC102206 is governed by a single dominant gene. Traditional mapping coupled with BSA-seq is used to map nematode resistance gene(s) using the BC<sub>1</sub>F<sub>1</sub> population derived from a cross of <italic>O. sativa</italic> cv. PR121 (S) and <italic>O. glaberrima</italic> acc. IRGC102206 (R). One major novel genomic region spanning a 3.0-Mb interval on chromosome 6 and two minor QTLs on chromosomes 2 and 4 are the potential genomic regions associated with rice root-knot nematode resistance. Within the QTL regions, 19 putative candidate genes contain 81 non-synonymous variants. The detected major candidate region could be fine mapped to accelerate marker-assisted breeding for root-knot nematode resistance in rice.</p>