AUTHOR=Li Songxue , Li Xiaoying , Zhang Shuang , Feng Yanan , Jia Tianshuang , Zhu Manning , Fang Lei , Gong Liping , Dong Shuang , Kong Xianchao , Wang Zhenzhen , Sun Litao 

TITLE=Association Between GSDMB Gene Polymorphism and Cervical Cancer in the Northeast Chinese Han Population

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.860727

DOI=10.3389/fgene.2022.860727

ISSN=1664-8021

ABSTRACT=<p><bold>Objective:</bold> The purpose of this study was to investigate the relationship between <italic>GSDMB</italic> gene polymorphism and genetic susceptibility to cervical cancer in the Han population in Northeast China.</p><p><bold>Methods:</bold> In this case–control study, the genotypes and alleles of rs8067378 in the <italic>GSDMB</italic> gene were analyzed by multiplex polymerase chain reaction (PCR) and next-generation sequencing methods in 482 cervical cancer (CC) patients, 775 cervical squamous intraepithelial lesion (SIL) patients, and 495 healthy women. The potential relationships between the SNP of the <italic>GSDMB</italic> gene with SIL and CC were analyzed by multivariate logistic regression analysis combined with 10,000 permutation tests.</p><p><bold>Results:</bold> In the comparison between the SIL group and the control group, the genotype and allele distribution frequencies of rs8067378 SNP of the <italic>GSDMB</italic> gene were statistically significant (<italic>p</italic> = 0.0493 and <italic>p</italic> = 0.0202, respectively). The allele distribution frequencies of rs8067378 were also statistically significant in the comparison between high-grade cervical squamous intraepithelial lesion (HSIL) and low-grade cervical squamous intraepithelial lesion (LSIL) groups with control group ( <italic>p</italic> = 0.0483 and <italic>p</italic> = 0.0330, respectively). Logistic regression analysis showed that after adjusting for age, the rs8067378 SNP of the <italic>GSDMB</italic> gene was significantly associated with the reduced risk of SIL under the dominant model (<italic>p</italic> = 0.0213, OR = 0.764, CI = 0.607–0.961) and the additive model (<italic>p</italic> = 0.0199, OR = 0.814, and CI = 0.684–0.968), and its mutant gene G may play a role in the progression of healthy people to LSIL and even HSIL as a protective factor. However, there was no significant association between cervical cancer and its subtypes with the control group (<italic>p</italic> &gt; 0.05). After 10,000 permutations, there was still no correlation that has provided evidence for the accuracy of our study.</p><p><bold>Conclusion:</bold> The results of this study showed that rs8067378 single nucleotide polymorphism of the <italic>GSDMB</italic> gene may reduce the risk of SIL and protect the susceptibility to cervical precancerous lesions in the Northeast Chinese Han population, but it has no significant correlation with the progression of cervical cancer.</p>