AUTHOR=Wang Daru , Wang Xun , Zhang Chunling , Yang Kuo , Wang Xinjie , Cui Jianying , Liu Dandan , You Chunxiang 

TITLE=Genome-wide Identification, Expression, and Functional Analysis of MdMSI Genes in Apples (Malus domestica Borkh.)

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.846321

DOI=10.3389/fgene.2022.846321

ISSN=1664-8021

ABSTRACT=<p>The multicopy suppressor of IRA (MSI) is a subfamily of WD40 repeat proteins, which is widely involved in plant growth and development. In order to explore the function of <italic>MdMSI</italic> members in abiotic stress, we identified eight <italic>MSI</italic> gene family members from the <italic>Malus</italic> × <italic>domestica</italic> reference genome. They were distributed on six chromosomes, and they had similar secondary and tertiary structures. We found a variety of regulatory elements in response to hormones and abiotic stress in <italic>MdMSI</italic> promoters. Through qRT-PCR analysis, it was revealed that <italic>MdMSIs</italic> were expressed in all tissues, especially in roots. The analysis results also revealed that the expression of <italic>MdMSIs</italic> was induced in varying degrees under salt, drought stress, and ABA treatments. Furthermore, we obtained the overexpression of <italic>MdMSI1-1</italic> transgenic apple calli and <italic>Arabidopsis</italic>. The overexpression of <italic>MdMSI1-1</italic> in calli and <italic>Arabidopsis</italic> played a negative regulatory role in salt stress response. Our work laid a foundation for further verifying the function of <italic>MSI</italic> genes under abiotic stress in apples.</p>