AUTHOR=Bhurta Ramesh , Hurali Deepak T. , Tyagi Sandhya , Sathee Lekshmy , Adavi B Sandeep , Singh Dalveer , Mallick Niharika , Chinnusamy Viswanathan , Vinod  , Jha Shailendra K. 

TITLE=Genome-Wide Identification and Expression Analysis of the Thioredoxin (Trx) Gene Family Reveals Its Role in Leaf Rust Resistance in Wheat (Triticum aestivum L.)

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.836030

DOI=10.3389/fgene.2022.836030

ISSN=1664-8021

ABSTRACT=<p>Bread wheat (<italic>Triticum aestivum</italic> L.; <italic>Ta</italic>) is the staple cereal crop for the majority of the world’s population. Leaf rust disease caused by the obligate fungal pathogen, <italic>Puccinia triticina</italic> L., is a biotrophic pathogen causing significant economic yield damage. The alteration in the redox homeostasis of the cell caused by various kinds of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in response to pathogenic infections is controlled by redox regulators. Thioredoxin (Trx) is one of the redox regulators with low molecular weight and is thermostable. Through a genome-wide approach, forty-two (42) wheat <italic>Trx</italic> genes (<italic>TaTrx</italic>) were identified across the wheat chromosome groups A, B, and D genomes containing 12, 16, and 14 <italic>Trx</italic> genes, respectively. Based on <italic>in silico</italic> expression analysis, 15 <italic>TaTrx</italic> genes were selected and utilized for further experimentation. These 15 genes were clustered into six groups by phylogenetic analysis. MicroRNA (miRNA) target analysis revealed eight different miRNA-targeted <italic>TaTrx</italic> genes. Protein–protein interaction (PPI) analysis showed <italic>TaTrx</italic> proteins interact with thioredoxin reductase, peroxiredoxin, and uncharacterized proteins. Expression profiles resulting from quantitative real-time PCR (qRT-PCR) revealed four <italic>TaTrx</italic> genes (<italic>TaTrx11-5A</italic>, <italic>TaTrx13-5B</italic>, <italic>TaTrx14-5D</italic>, and <italic>TaTrx15-3B</italic>) were significantly induced in response to leaf rust infection. Localization of ROS and its content estimation and an assay of antioxidant enzymes and expression analysis suggested that <italic>Trx</italic> have been involved in ROS homeostasis at span 24HAI-72HAI during the leaf rust resistance.</p>