AUTHOR=Huzar Jared , Kim Hannah , Kumar Sudhir , Miura Sayaka 

TITLE=MOCA for Integrated Analysis of Gene Expression and Genetic Variation in Single Cells

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.831040

DOI=10.3389/fgene.2022.831040

ISSN=1664-8021

ABSTRACT=<p>In cancer, somatic mutations occur continuously, causing cell populations to evolve. These somatic mutations result in the evolution of cellular gene expression patterns that can also change due to epigenetic modifications and environmental changes. By exploring the concordance of gene expression changes with molecular evolutionary trajectories of cells, we can examine the role of somatic variation on the evolution of gene expression patterns. We present Multi-Omics Concordance Analysis (MOCA) software to jointly analyze gene expressions and genetic variations from single-cell RNA sequencing profiles. MOCA outputs cells and genes showing convergent and divergent gene expression patterns in functional genomics.</p>