AUTHOR=Rui Cun , Chen Xiugui , Xu Nan , Wang Jing , Zhang Hong , Li Shengmei , Huang Hui , Fan Yapeng , Zhang Yuexin , Lu Xuke , Wang Delong , Gao Wenwei , Ye Wuwei 

TITLE=Identification and Structure Analysis of KCS Family Genes Suggest Their Reponding to Regulate Fiber Development in Long-Staple Cotton Under Salt-Alkaline Stress

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.812449

DOI=10.3389/fgene.2022.812449

ISSN=1664-8021

ABSTRACT=<p>Plant 3-ketoacyl-CoA synthase (<italic>KCS</italic>) gene family catalyzed a β ketoacyl-CoA synthase, which was the rate-limiting enzyme for the synthesis of very long chain fatty acids (VLCFAs). <italic>Gossypium barbadense</italic> was well-known not only for high-quality fiber, which was perceived as a cultivated species of <italic>Gossypium</italic>. In this study, a total of 131 <italic>KCS</italic> genes were identified in four cotton species, there were 38, 44, 26, 23 <italic>KCS</italic> genes in the <italic>G. barbadense</italic>, the <italic>G. hirsutum</italic>, the <italic>G. arboreum</italic> and <italic>G. raimondii</italic>, respectively. The gene structure and expression pattern were analyzed. <italic>GBKCS</italic> genes were divided into six subgroups, the chromosome distribution of members of the family were mapped. The prediction of cis-acting elements of the <italic>GBKCS</italic> gene promoters suggested that the <italic>GBKCS</italic> genes may be involved in hormone signaling, defense and the stress response. Collinearity analysis on the <italic>KCS</italic> genes of the four cotton species were formulated. Tandem duplication played an indispensable role in the evolution of the <italic>KCS</italic> gene family. Specific expression analysis of 20 <italic>GBKCS</italic> genes indicated that <italic>GBKCS</italic> gene were widely expressed in the first 25 days of fiber development. Among them, <italic>GBKCS3</italic>, <italic>GBKCS8</italic>, <italic>GBKCS20</italic>, <italic>GBKCS34</italic> were expressed at a high level in the initial long-term level of the <italic>G. barbadense</italic> fiber. This study established a foundation to further understanding of the evolution of <italic>KCS</italic> genes and analyze the function of <italic>GBKCS</italic> genes.</p>