AUTHOR=Guo Liping , You Chen , Zhang Hanghang , Wang Yukun , Zhang Rui 

TITLE=Genome-wide analysis of NBS-LRR genes in Rosaceae species reveals distinct evolutionary patterns

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.1052191

DOI=10.3389/fgene.2022.1052191

ISSN=1664-8021

ABSTRACT=<p>The nucleotide-binding site and leucine-rich repeat (<italic>NBS-LRR</italic>) genes, one of the largest gene families in plants, are evolving rapidly and playing a critical role in plant resistance to pathogens. In this study, a genome-wide search in 12 Rosaceae genomes screened out 2188 <italic>NBS-LRR</italic> genes, with the gene number varied distinctively across different species. The reconciled phylogeny revealed 102 ancestral genes (7 <italic>RNLs</italic>, 26 <italic>TNLs</italic>, and 69 <italic>CNLs</italic>), which underwent independent gene duplication and loss events during the divergence of the Rosaceae. The <italic>NBS-LRR</italic> genes exhibited dynamic and distinct evolutionary patterns in the 12 Rosaceae species due to independent gene duplication/loss events, which resulted the discrepancy of <italic>NBS-LRR</italic> gene number among Rosaceae species. Specifically, <italic>Rubus occidentalis</italic>, <italic>Potentilla micrantha</italic>, <italic>Fragaria iinumae</italic> and <italic>Gillenia trifoliata</italic>, displayed a “first expansion and then contraction” evolutionary pattern; <italic>Rosa chinensis</italic> exhibited a “continuous expansion” pattern; <italic>F. vesca</italic> had a “expansion followed by contraction, then a further expansion” pattern, three <italic>Prunus</italic> species and three <italic>Maleae</italic> species shared a “early sharp expanding to abrupt shrinking” pattern. Overall, this study elucidated the dynamic and complex evolutionary patterns of <italic>NBS-LRR</italic> genes in the 12 Rosaceae species, and could assist further investigation of mechanisms driving these evolutionary patterns.</p>