AUTHOR=Yi Chuncheng , Li Tiandong , Shen Yajing , Wang Peng , Dai Liping , Shi Jianxiang , Wang Keyan , Sun Changqing , Ye Hua 

TITLE=Polymorphisms of nucleotide excision repair genes associated with colorectal cancer risk: Meta-analysis and trial sequential analysis

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.1009938

DOI=10.3389/fgene.2022.1009938

ISSN=1664-8021

ABSTRACT=<p><bold>Background:</bold> Reduced DNA repair capacity in nucleotide excision repair (NER) pathways owing to genetic variant may influence cancer susceptibility. According to published studies, variants of NER genes associations with colorectal cancer (CRC) risk were inconclusive. Thus, this meta-analysis aimed to explore the possible association. A trial sequence analysis (TSA) analysis was performed to control the risk of false positive or false negative.</p><p><bold>Methods:</bold> PubMed, Web of Science, Embase, Cochrane Library, China National Knowledge Network (CNKI), Wanfang Database and Scientific and Technical Journal Database (VIP) were searched to identify relative studies until April 2022. The association was assessed by odds ratio (OR) in Allele, homozygous, heterozygous, dominant, recessive, and over-dominant models. In addition, Begg’s and Egger’s tests, sensitivity analysis, subgroup analysis and TSA analysis were performed.</p><p><bold>Results:</bold> A total of 29 studies were eventually included in the meta-analysis, including 12,153 CRC patients and 14,168 controls. It showed that excision and repair cross complementary group 1 (<italic>ERCC1</italic>) rs11615 CC genotype decreased the risk of CRC, compared with TT genotype (CC vs. TT: OR = 0.816, 95% CI = 0.673–0.990, <italic>p</italic> = 0.039). For <italic>ERCC1</italic> rs3212986, the significant impact was detected on increased the risk of CRC in the allele (OR = 1.267, 95% CI = 1.027–1.562, <italic>p</italic> = 0.027), homozygous (OR = 1.805, 95% CI = 1.276–2.553, <italic>p</italic> = 0.001), dominant (OR = 1.214, 95% CI = 1.012–1.455, <italic>p</italic> = 0.037) and recessive (OR = 1.714, 95% CI = 1.225–2.399, <italic>p</italic> = 0.002) models, especially in the Asian population. The results revealed the association of <italic>ERCC2</italic> rs1799793 A allele with a higher risk of CRC (A vs. G: OR = 1.163, 95% CI = 1.021–1.325, <italic>p</italic> = 0.023). It also showed that <italic>ERCC5</italic> rs17655 increased CRC risk in the allele (OR = 1.104, 95% CI = 1.039–1.173, <italic>p</italic> = 0.001), homozygous (OR = 1.164, 95% CI = 1.018–1.329, <italic>p</italic> = 0.026), heterozygous (OR = 1.271, 95% CI = 1.018–1.329, <italic>p</italic> &lt; 0.001), dominant (OR = 1.241, 95% CI = 1.135–1.358, <italic>p</italic> &lt; 0.001) and over-dominant (OR = 0.828, 95% CI = 0.762–0.900, <italic>p</italic> &lt; 0.001) models, especially among Asians.</p><p><bold>Conclusion:</bold> This meta-analysis based on current evidence suggests that the significant association was observed between <italic>ERCC1</italic> rs11615, <italic>ERCC1</italic> rs3212986, <italic>ERCC2</italic> rs1799793, and <italic>ERCC5</italic> rs17655 and CRC susceptibility. However, given the limited sample size and the influence of genetic background, studies of a larger scale and well-designed are required to confirm the results.</p>