AUTHOR=Dubey Namo , Chaudhary Anjali , Singh Kunal 

TITLE=Genome-Wide Analysis of TIR-NBS-LRR Gene Family in Potato Identified StTNLC7G2 Inducing Reactive Oxygen Species in Presence of Alternaria solani

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.791055

DOI=10.3389/fgene.2021.791055

ISSN=1664-8021

ABSTRACT=<p>Resistance gene analogs (RGAs) comprising NBS-LRR gene family members are considered prominent candidates in the development of disease-resistant genotypes. NBS-LRR gene family comprised a very large number of genes; therefore, members of one subfamily TIR-NBS-LRR (TNL) are identified in the present study from <italic>Solanum tuberosum</italic> genome, followed by their bioinformatics characterization. The study identified a total of 44 genes encoding 60 TNL transcripts with two prominent clusters at chromosome 1 and chromosome 11. Expression analysis of 14 TNL genes after <italic>Alternaria solani</italic> infection at 1, 2, 3, 5, and 7 days post inoculation in two disease-tolerant varieties, Kufri Jyoti and Kufri Pukhraj, and one relatively susceptible variety, Kufri Chandramukhi, showed differential expression of many genes including a high expression (&gt;15-fold) of <italic>StTNLC6G2T1</italic> and <italic>StTNLC11G9T1</italic>. Functional characterization of one such gene, <italic>StTNLC7G2</italic>, reveals involvement in the generation of reactive oxygen species under <italic>A. solani</italic> attack, implicating its putative role in plant defense <italic>via</italic> hypersensitive response.</p>