AUTHOR=Huang Jia-Bin , Hu Bin-Bin , He Rong , He Lian , Zou Chen , Man Chang-Feng , Fan Yu 

TITLE=Analysis of N6-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.787800

DOI=10.3389/fgene.2021.787800

ISSN=1664-8021

ABSTRACT=<p><bold>Background:</bold> From previous studies, we found that there are more than 100 types of RNA modifications in RNA molecules. m<sup>6</sup>A methylation is the most common. The incidence rate of adenocarcinoma of the esophagogastric junction (AEG) at home and abroad has increased faster than that of stomach cancer at other sites in recent years. Here, we systematically analyze the modification pattern of m<sup>6</sup>A mRNA in adenocarcinoma at the esophagogastric junction.</p><p><bold>Methods:</bold> m<sup>6</sup>A sequencing, RNA sequencing, and bioinformatics analysis were used to describe the m<sup>6</sup>A modification pattern in adenocarcinoma and normal tissues at the esophagogastric junction.</p><p><bold>Results:</bold> In AEG samples, a total of 4,775 new m<sup>6</sup>A peaks appeared, and 3,054 peaks disappeared. The unique m6A-related genes in AEG are related to cancer-related pathways. There are hypermethylated or hypomethylated m<sup>6</sup>A peaks in AEG in differentially expressed mRNA transcripts.</p><p><bold>Conclusion:</bold> This study preliminarily constructed the first m<sup>6</sup>A full transcriptome map of human AEG. This has a guiding role in revealing the mechanism of m<sup>6</sup>A-mediated gene expression regulation.</p>