AUTHOR=Rehman Shamsur , Rashid Arif , Manzoor Muhammad Aamir , Li Lingling , Sun Weibo , Riaz Muhammad Waheed , Li Dawei , Zhuge Qiang 

TITLE=Genome-Wide Evolution and Comparative Analysis of Superoxide Dismutase Gene Family in Cucurbitaceae and Expression Analysis of Lagenaria siceraria Under Multiple Abiotic Stresses

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.784878

DOI=10.3389/fgene.2021.784878

ISSN=1664-8021

ABSTRACT=<p>Superoxide dismutase (SOD) is an important enzyme that serves as the first line of defense in the plant antioxidant system and removes reactive oxygen species (ROS) under adverse conditions. The SOD protein family is widely distributed in the plant kingdom and plays a significant role in plant growth and development. However, the comprehensive analysis of the <italic>SOD</italic> gene family has not been conducted in Cucurbitaceae. Subsequently, 43 <italic>SOD</italic> genes were identified from Cucurbitaceae species [<italic>Citrullus lanatus</italic> (watermelon), <italic>Cucurbita pepo</italic> (zucchini), <italic>Cucumis sativus</italic> (cucumber), <italic>Lagenaria siceraria</italic> (bottle gourd), <italic>Cucumis melo</italic> (melon)]. According to evolutionary analysis, <italic>SOD</italic> genes were divided into eight subfamilies (I, II, III, IV, V, VI, VII, VIII). The gene structure analysis exhibited that the <italic>SOD</italic> gene family had comparatively preserved exon/intron assembly and motif as well. Phylogenetic and structural analysis revealed the functional divergence of Cucurbitaceae <italic>SOD</italic> gene family. Furthermore, microRNAs 6 miRNAs were predicted targeting 3 <italic>LsiSOD</italic> genes. Gene ontology annotation outcomes confirm the role of <italic>LsiSODs</italic> under different stress stimuli, cellular oxidant detoxification processes, metal ion binding activities, SOD activity, and different cellular components. Promoter regions of the <italic>SOD</italic> family revealed that most <italic>cis</italic>-elements were involved in plant development, stress response, and plant hormones. Evaluation of the gene expression showed that most <italic>SOD</italic> genes were expressed in different tissues (root, flower, fruit, stem, and leaf). Finally, the expression profiles of eight <italic>LsiSOD</italic> genes analyzed by qRT-PCR suggested that these genetic reserves responded to drought, saline, heat, and cold stress. These findings laid the foundation for further study of the role of the <italic>SOD</italic> gene family in Cucurbitaceae. Also, they provided the potential for its use in the genetic improvement of Cucurbitaceae.</p>